电针对睡眠剥夺大鼠行为学和脾脏IL-1β、IL-6和TNF-α的影响

Effects of electro-acupuncture on behavior change and cytokines IL-1β, IL-6, TNF-α in the spleen of sleep deprivation rats

关键词:  电针  睡眠剥夺  细胞因子

Key words:  electro-acupuncture  sleep deprivation  cytokine

摘要:

[目的] 观察电针对睡眠剥夺大鼠行为学和脾脏白细胞介素(IL)-1β、IL-6和肿瘤坏死因子-α(TNF-α)mRNA表达的影响。[方法] 将32只Wistar雄性大鼠随机分为空白对照组、模型组、安定组和电针组。对照组未做任何处理,其余3组给予注射氯苯丙氨酸(PCPA)睡眠剥夺后,安定组给予腹腔注射安定,电针组给予电针百会、神庭,每组每日治疗1次,连续5 d后行旷场实验并采用逆转录-聚合酶链反应(RT-PCR)检测大鼠脾脏IL-1β、IL-6和TNF-α。[结果] 与对照组相比,模型组大鼠旷场实验水平得分、垂直得分和中央格停留时间明显升高,差异具有统计学意义(P<0.05);与模型组相比,安定组和电针组旷场实验水平得分、垂直得分和中央格停留时间明显降低,差异具有统计学意义(P<0.05)。与对照组相比,模型组IL-1β和TNF-α含量显著升高,差异具有统计学意义(P<0.01),IL-6无统计学差异(P>0.05);与模型组相比,安定组和电针组IL-1β和TNF-α含量显著降低,差异具有统计学意义(P<0.05),IL-6无统计学差异(P>0.05)。[结论] 电针百会、神庭既能明显改善睡眠剥夺症状,又能很好控制IL-1β、IL-6和TNF-α的表达,解除失眠对机体的损害。

Abstract:

[Objective] To observe the effect of electro-acupuncture on behavior change and the cytokines of IL-1β, IL-6, TNF-α in the spleen of sleep deprivation rats to investigate the mechanism of electro-acupuncture treatment in insomnia. [Methods] Thirty-two Wistar rats with SPF level were randomized into the control group, model group, diazepam group and electro-acupuncture group. The sleep deprivation rats were established by DL-P-Chlorophenylalanine (PCPA) abdominal injection. The diazepam group were treated by diazepam, and the electro-acupuncture group were treated by electro-acupuncture with Baihui (GV20) and Shenting (GV24) acupoints. Behavior change and the cytokines of sleep deprivation rats were observed and analyzed by real time-PCR after 5 days continuously treatment. [Results] Comparison with the control group in the open field test, the crossing scores, the rearing scores and the pace time in center of the rats were significantly up-regulated in the model group(P<0.05). On the other hand, all of those scores mentioned above were significantly increased in the group of diazepam and electro-acupuncture(P<0.05). In the results of real time-PCR, the IL-1β and the TNF-α expression levels were remarkably higher in the model group than in the control group(P<0.01). Furthermore, the IL-1β and the TNF-α expression levels in the diazepam group and the electro-acupuncture group were Significantly down-regulated(P<0.05). But there was no obviously results in each group with the level of IL-6 (P>0.05). [Conclusion] The electro-acupuncture can effectively treat the symptoms of Insomnia, and it also can proper control the level of cytokines.