基于IL-7的甘草多糖抗肿瘤机制的研究

Study on the anti-tumor effect mechanism of Gancao polysaccharide on production of IL-7

关键词:  甘草多糖  小肠黏膜上皮细胞  抗肿瘤  白细胞介素-7

Key words:  Gancao polysaccharide  small intestinal mucosal epithelial cell  anti-tumor  IL-7

摘要:

[目的] 明确甘草多糖抗肿瘤作用及其作用机制。[方法] 建立CT-26细胞荷瘤小鼠动物模型,随机分为正常组、模型组、甘草多糖组(甘草多糖灌胃500mg/kg)、香菇多糖组(阳性药组,香菇多糖灌胃195mg/kg)和白细胞介素-7(IL-7)抗体阻断组(甘草多糖灌胃500 mg/kg,IL-7抗体与 0、5、10 d腹腔注射0.4 mg/kg).连续给药14 d,计算小鼠肿瘤质量、抑瘤率、脾脏指数、胸腺指数;检测小肠黏膜上皮细胞IL-7mRNA表达情况和血清中IL-7含量。[结果] 灌胃给予甘草多糖14 d后,甘草多糖可以显着降低荷瘤小鼠肿瘤质量,其抑瘤率为21.56%;显着提高小鼠体质量和胸腺指数,改善其生活质量;提高小肠黏膜上皮细胞IL-7mRNA表达和血清中IL-7含量;IL-7抗体阻断后,其抑瘤率和胸腺指数显着降低,小肠黏膜上皮细胞IL-7mRNA虽高表达,但血清中IL-7含量低于试剂盒检测限。[结论] 甘草多糖具有显着的抗肿瘤作用,其抗肿瘤机制可能与提高小肠黏膜上皮细胞分泌IL-7有关。

Abstract:

[Objective] To elucidate the mechanism of Gancao polysaccharide (GP) on anti-tumor. [Methods] CT-26 cells were used to set up the tumor-bearing model in mice. The mice were respectively divided into following groups: normal group, model group, GP group (GP i.g, 500 mg/kg), letinan group (letinan i.g, 195 mg/kg) and IL-7 antibody group (GP i.g, 500 mg/kg, IL-7antibody i.p,.0.4 mg/kg on day 0, 5 and 10). After administration of GP for 14 days, the tumor, spleen and thymus were weighted to measure the tumor inhibition rat, spleen index and thymus index. [Results] After the administration of GP for 14 days, the tumor inhibition rate in GP group was 21.56%.The IL-7 gene expression in GP group was upregulated and the serum level of IL-7 was increased in GP group. The tumor inhibition rate and thymus index were decreased in IL-7 antibody group followed by the significant lower IL-7 level in serum in spite of the IL-7 gene expressed in intestine. [Conclusion] This study demonstrated that GP exert significant anti-tumor effect and the mechanism behind the anti-tumor effect may relate to the IL-7 production of intestinal epithelial cells.