HPLC法测定稳心颗粒中三七皂苷R1、人参皂苷Rg1、Rb1、Rd与党参炔苷的含量

Determination of Notoginsenoside R1, Ginsenoside Rg1, Rb1, Rd and Lobetyolin in Wenxin Keli by HPLC

关键词:  稳心颗粒  高效液相色谱法  三七皂苷R₁  人参皂苷Rg₁  党参炔苷  人参皂苷Rb₁  人参皂苷Rd

Key words:  Wenxin Keli  HPLC  Notoginsenoside R₁  Ginsenoside Rg₁  Lobetyolin Ginseno-side Rb₁  Ginsenoside Rd

摘要:

[目的] 建立高效液相法（HPLC）同时测定稳心颗粒中三七皂苷R₁、人参皂苷Rg₁、Rb₁、Rd与党参炔苷的含量。[方法] 采用Agilent1260 DAD高效液相色谱仪，Amethyst C₁₈色谱柱（4.6 mm×250 mm，4μm），乙腈-水为流动相，检测波长为210 nm，流速1 mL/min。[结果] 三七皂苷R₁对照品在8~247 mg/L线性关系良好，平均回收率为102.69%，RSD=2.74%（n=6）；人参皂苷Rg₁对照品在14~422 mg/L线性关系良好，平均回收率为98.72%，RSD=1.85%（n=6）；党参炔苷对照品在1.5~42 mg/L线性关系良好，平均回收率为97.69%，RSD=1.94%（n=6）；人参皂苷Rb₁对照品在2.65~847 mg/L线性关系良好，回收率为102.11%，RSD=1.96%（n=6）；人参皂苷Rd对照品在8~255 mg/L线性关系良好，平均回收率为99.66%，RSD=2.49%（n=6）。[结论] 本实验中三七皂苷R₁、人参皂苷Rg₁、Rb₁、Rd和党参炔苷的含量测定方法稳定可靠，可作为稳心颗粒中三七皂苷R₁、人参皂苷Rg₁、Rb₁、Rd与党参炔苷含量测定方法。

Abstract:

[Objective] To establish a HPLC method for simulataneous determination of Notoginsenoside R₁ (1), Ginsenoside Rg₁ (2), lobetyolin (3), Ginsenoside Rb₁ (4), Ginsenoside Rd (5) in Wenxin Keli by HPLC.[Methods] The HPLC was conducted on Amethyst C₁₈ column (4.6 mm×250 mm, 4 μm) with acetonitrile-water solution as themobile phase. The detection wavelength was 210 nm, and flow rate was 1 mL/min.[Results] Linearity of each standard was established within the concentration range of 8~247 mg/L for 1, 14~422 mg/L for 2, 1.5~42 mg/L for 3, 26.5~847 mg/L for 4 and 8~255 mg/L for 5. The average recovery(n=6) of the compound 1-5 was 102.69% with RSD of 2.74%, 98.72% with RSD of 1.85%, 97.69% with RSD of 1.94%, 102.11% with RSD of 1.96%, 99.66% with RSD of 2.49% respectively.[Conclusion] The treatment of samples was stable and reliable. The method can be used for determination of notoginsenoside R₁, lobetyolin, ginsenoside Rg₁, Rb₁ and Rd in Wenxin Keli.