消痞方对化疗后痞满证大鼠胃排空率及胃肌层Cajal间质细胞的影响

Effect of Xiaopi prescription on interstitial cells and the gastric emptying rate of gastric muscle layer Cajal in rats of distention and fullness after chemotherapy

关键词:  消痞方  化疗  痞满证  Cajal间质细胞

Key words:  Xiaopi prescription  chemotherapy  distention and fullness syndrome  ICC

摘要:

[目的] 观察消痞方对化疗后痞满证大鼠胃排空率及Cajal间质细胞结构及功能的影响，探讨化疗所致痞满证机制。[方法] 以Wistar大鼠（60只）为研究对象，随机分为空白组、模型组、西药组、消痞方高剂量组、消痞方中剂量组、消痞方低剂量组，每组10只大鼠。除空白组外，其余5组采用尾静脉注射化疗药物建立大鼠脾虚证模型。空白组和模型组给予生理盐水灌胃，消痞方低剂量组、中剂量组与高剂量组分别给予浓度100%、150%、200%的煎剂灌胃，西药组给予西沙必利灌胃，共给药7 d。观察各组大鼠日进食量改变，检测胃排空率，采用透射电镜观察胃起搏区Cajal间质细胞（ICC）的形态特征和数量。[结果] 模型组大鼠日进食量及胃排空率较空白组显著降低（P<0.05），ICC数量减少，结构破坏，细胞器减少，与神经末梢及平滑肌细胞之间连接破坏。消痞方高剂量组大鼠进食量及胃排空明显增加，与模型组比较差异具有统计学意义（P<0.05），ICC结构基本恢复正常，新生增多。西药组大鼠进食量及胃排空也可见增加，但部分ICC结构仍可见破坏。消痞方低剂量及中剂量组较模型组差异无统计学意义。[结论] 对ICC结构的影响可能是消痞方治疗化疗后痞满证的机制之一。

Abstract:

[Objective] To observe Xiaopi prescription on interstitial cells and the gastric emptying rate of gastric muscle layer Cajal in rats of distention and fullness after chemotherapy. To explore the mechanism of distention and fullness induced by chemotherapy. [Methods] Wistar rats (n=60) as the object of study randomly divided into blank group, model group, Western medicine group, Xiaopi prescription high dosage group, Xiaopi prescription middle dosage group, Xiaopi prescription low dosage group, 10 rats in each group. Except for the blank group, the other 5 groups were injected with the tail vein for the establishment of the spleen deficiency syndrome model of rats. Blank group and model group were given physiological saline gavage. Xiaopi prescription high dosage group, Xiaopi prescription middle dosage group, Xiaopi prescription low dosage group were given concentration of 100%, 150% and 200% decoction orally. The Western medicine group was given cisapride intragastric administration, administration for 7 days. The daily feed intake of rats was observed. The gastric emptying rate was detected, and the morphology and quantity of ICC in the gastric pacing region were observed by transmission electron microscope. [Results] The eating and gastric emptying rate in the model group decreased significantly compared with the control group (P<0.05), and the number of ICC, structural damage, fewer organelles, between the nerve endings and smooth muscle cells were reduced, and the connection was damaged. The food intake and gastric emptying in Xiaopi prescription high dosage group rats significantly increased compared with model group, and the difference had statistical significance (P<0.05). ICC structure returned to normal, and new increased. The food intake and gastric emptying in Western medicine group were increased, but part of the structure of the ICC still visible damaged. Xiaopi prescription low dose and middle dose group compared with the model group of them, which had no significant difference. [Conclusion] The effect of ICC may be one of the mechanisms of Xiaopi prescription in the treatment of epigastric fullness after chemotherapy.