肝肺同治祛风方治疗咳嗽变异性哮喘模型大鼠的疗效观察

Observation on the curative effect of eliminating wind prescription of treatment lung and liver together in CVA rats

关键词:  咳嗽变异性哮喘  大鼠  肝郁肺虚  肝肺同治  IgE

Key words:  cough variant asthma  rat  liver depression and lung deficiency  treating lung and liver together  IgE

摘要:

[目的] 观察肝肺同治祛风法对咳嗽变异性哮喘（CVA）大鼠模型的疗效。[方法] 将72只大鼠分为正常对照组、模型对照组、激素吸入组、清肺祛风组、清肝熄风组、肝肺同治组，制备咳嗽变异性哮喘肝郁肺虚病证结合大鼠模型，观察各组大鼠一般状态、肺支气管病理组织形态、支气管肺泡灌洗液（BALF）嗜酸性粒细胞计数及测定血清IgE水平的变化。[结果] 与正常对照组比较，模型对照组CVA大鼠支气管管腔狭窄，黏膜增厚，支气管壁及可见大量嗜酸性粒细胞浸润，BALF涂片EOS计数显著升高，血清中IgE的含量显著升高，与正常对照组比较均有显著性差异（P<0.01）。激素治疗组及各中药治疗组，各项指标均有显著改善，与模型组比较有显著性差异（P<0.01），以激素治疗组及肝肺同治组指标改善最为显著。[结论] 肝肺同治法可改善肝郁肺虚的中医证候，降低CVA模型大鼠慢性气道炎症反应，调整免疫异常。

Abstract:

[Objective] To investigate the curative effect of eliminating wind prescription of treatment lung and liver together in CVA rats. [Methods] The combination of disease and syndrome animal model of CVA rats was established by traditional Chinese medicine (TCM) etiology and Western medicine etiology pathology, then they were randomly divided into normal group, model group, hormone inhaling group, clear lung heat and eliminating wind evil method group, clear liver to stop wind group, both lung and Liver treatment group. Observation the rat general state, spontaneous activity, cough response, bronchial lung pathology organization form, bronchoalveolar lavage fluid (BALF) eosinophil count and serum IgE. [Results] Comparison with normal rats, the bronchial lumen of the model group rats were stenosis,mucosal thickening,numerous eosinophils infiltration on bronchial wall, the EOS counting was significant rise in BALF smear, the content of serum IgE was significantly increased(P<0.01). Comparison with model rats, bronchial lung pathology, the EOS counting and serum IgE content of hormone inhaling group and TCM treatment group were significantly increased(P<0.01), especially the hormone inhaling group and treating both lung and liver group. [Conclusion] Treating lung and liver together could significantly improved the TCM syndrome of liver depression and lung deficiency, reduced the chronic airway inflammation of CVA model rats and regulate the immune abnormalities.