| 摘要: |
| [目的] 建立高效液相色谱法（HPLC-UV）同时测定南葶苈子中6种成分（槲皮素-3-O-β-D-吡喃葡萄糖-7-O-β-D-龙胆双糖苷，槲皮素-3-O-β-D-吡喃葡萄糖苷，异鼠李素-3-O-β-D-吡喃葡萄糖苷，槲皮素，山奈酚，异鼠李素）的含量测定方法，为南葶苈子质量评价提供技术支撑。[方法] 采用反相高效液相色谱法，Agilent Eclipse Plus C18色谱柱（4.6 mm×100 mm，1.8 μm），流动相为乙腈-0.1%磷酸水溶液，梯度洗脱，流速0.3 mL/min，柱温为30℃，检测波长为265 nm。[结果] 6种成分线性关系良好，加样回收率范围是99.1%~103.0%。[结论] 本研究建立的南葶苈子中6种成分含量测定方法准确、可靠，可用于南葶苈子的质量控制。 |
| 关键词: 南葶苈子 HPLC-UV 含量测定 |
| DOI：10.11656/j.issn.1672-1519.2016.12.12 |
| 分类号: |
| 基金项目:中药标准饮片制备技术规范制定（2014FY111100）；天津市高等学校创新团队培养计划资助（TD12-5033）。 |
|
| Simultaneous determination of six chemical components for quality control of Semen Descurainiae by HPLC-UV |
|
JIANG Yan1, YIN Xue-yan2, LI Jin1, WANG Hui2, DOU Zhi-ying2, CHANG Yan-xu1
|
|
1.Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Traditional Chinese Medicine College, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
|
| Abstract: |
| [Objective] An HPLC-UV method was established for simultaneous determination of the content of six components (quercetin-3-O-β-D-glucise-7-O-β-D-gentiobiosiden,quercetin-3-O-β-D-glucoside,isorhamnetin-3-O-β-D-glucoside,quercetin,kaempferol and isorhamnetin) in Semen Descurainiae in order to provide a theoretical basis for its quality assessment.[Methods] The analysis was performed on Agilent eclipse plus C18 column (4.6 mm×100 mm and 1.8 μm).Acetonitrile -0.1% phosphoric acid water solution was used as mobile phase at flow rate of 0.3 mL/min.The column temperature was 30℃ and the detection wavelength was set at 265 nm.[Results] Six components were well separated with good linear relationship.The recoveries were 99.1%~103.0%.[Conclusion] The accurate and reliable HPLC-UV method was established and could be used for quality evaluation of Semen Descurainiae. |
| Key words: Semen Descurainiae HPLC-UV content determination |
