桂皮醛对滑膜成纤维细胞增殖、凋亡及周期影响的实验研究

Experimental research on the influence of cinnamic aldehyde on the proliferation, apoptosis and cell cycle of fibroblast-like synovial cells

关键词:  骨关节炎  桂皮醛  滑膜成纤维细胞  增殖  流式细胞术

Key words:  osteoarthritis  cinnamic aldehyde  fibroblast-like synovial cell  proliferation  flow cytometry

摘要:

[目的]研究不同浓度的桂皮醛（CA）对滑膜成纤维细胞（FLS）的增殖、凋亡及细胞周期的影响，进而探讨CA对骨关节炎滑膜炎症的调控机制。[方法]不同浓度的CA分别作用于LPS诱导后的FLS，通过四甲基偶氮唑盐微量酶反应比色法（MTT）、流式细胞术（FCM）对其增殖、凋亡和周期进行测定。[结果]低浓度的CA可轻度促进FLS的生长，较高浓度CA则可显著降低FLS的存活率（P<0.05），呈浓度依赖性。LPS可减少FLS的凋亡率，缩短G1期、延长S期，可用来制备炎症模型。与对照组相比，CA高浓度组与低浓度组均能是Q2和Q4区细胞比率明显增加（P<0.05），延长G1期并缩短S期（P<0.05），诱导FLS凋亡及抑制FLS的增殖，尤其是CA高浓度组作用更加显著。[结论]不同浓度的CA对FLS的生长均有一定的抑制作用，其中CA高浓度组的抑制作用更强，这可能是CA抗炎作用的潜在机制。

Abstract:

[Objective] To investigate the influence of different concentrations of cinnamic aldehyde (CA) on the proliferation, apoptosis and cell cycle of fibroblast-like synovial cells(FLS), and then explore the regulatory mechanism of CA to the synovial inflammation in osteoarthritis.[Methods] Different concentrations of CA were intervened on LPS-induced FLS respectively, and the proliferation, apoptosis and cell cycle were measured through Methylthiazolyldiphenyl-tetrazolium bromide assay (MTT) and flow cytometry(FCM).[Results] Low concentration of CA could promote the growth of FLS mildly, while high concentration of CA could significantly reduce the survival rate (P<0.05) in concentration-dependent manner. LPS could reduce the apoptosis rate of FLS, shorten the G1 phase and extend the S phase, it meant that the inflammatory model was successfully established. Compared with the control group, both high and low concentration of CA groups could obviously increase the ratio of cells in the areas of Q2 and Q4 (P<0.05), and extend the G1 phase and S phase (P<0.05) to induct the apoptosis and inhibit the proliferation of FLS, especially the effect of high concentration of CA group was more significant.[Conclusion] Different concentrations of CA has certain inhibition for the growth of FLS, and the high concentration of CA group has stronger inhibitory effect, which may be the potential mechanism of CA's anti-inflammatory effects.