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| 黄花夹竹桃叶中总强心苷的快速提取及含量测定研究 |
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温时媛1,2, 陈燕燕1,2, 李晓男1,2, 王跃飞1, 姜苗苗1
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1.天津中医药大学, 天津市现代中药重点实验室, 天津 300193;2.天津国际生物医药联合研究院, 中药新药研发中心, 天津 300457
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| 摘要: |
| [目的]研究黄花夹竹桃叶子中总强心苷的快速提取方法及含量测定方法。[方法]运用1H-NMR对总强心苷进行含量测定，对甲醇冷浸、70%乙醇加热回流、70%乙醇冷浸、95%乙醇温浸4种提取工艺进行筛选。[结果]95%乙醇温浸提取的强心苷量最多。[结论]95%乙醇温浸提取工艺是提取黄花夹竹桃叶中总强心苷的一种较佳的快速提取方法，并且测定总强心苷的含量可以采用1H-NMR技术。 |
| 关键词: 黄花夹竹桃 强心苷 提取工艺 1H-NMR |
| DOI：10.11656/j.issn.1672-1519.2017.01.14 |
| 分类号: |
| 基金项目:天津市高等学校科技发展基金计划项目（20130202）天津市科技特派员项目（15JCTPJC60000）。 |
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| Study on a rapid extraction method and content determination method of total cardiac glycosides from leaves of Thevetia peruviana |
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WEN Shi-yuan1,2, CHEN Yan-yan1,2, LI Xiao-nan1,2, WANG Yue-fei1, JIANG Miao-miao1
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1.Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Research and Development Center of Traditional Chinese Medicine Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China
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| Abstract: |
| [Objective] To study rapid extraction method and content determination method of total cardiac glycosides from leaves of Thevetia peruviana.[Methods] Using 1H-NMR to content determination of cardiac glycosides, 4 kinds of extraction process, the methanol soaking, 70% ethanol heating refluxing, 70% ethanol soaking, 95% ethanol gentle soaking, were screened.[Results] The 95% ethanol gentle soaking method extracted the highest amount of cardiac glycosides.[Conclusion] The extraction process of 95% ethanol gentle soaking is a better fast extraction method for extracting total cardiac glycoside from leaves of Thevetia peruviana, and content determination of cardiac glycosides can be employed by the 1H-NMR. |
| Key words: Thevetia peruviana cardiac glycoside extraction process 1H-NMR |
