| 本文已被：浏览 2404次 下载 2008次 |
码上扫一扫！ |
|
|
| 藤黄酸纳米结构脂质载体的制备及抗肿瘤作用初步评价 |
|
黄瑞1,2, 郭盼1,2, 刘勃缨1,2, 丁苗苗1,2, 张兵1,2, 张丽娜1,2, 刘志东1,2
|
|
1.天津中医药大学, 现代中药发现与制剂技术教育部工程中心, 天津 300193;2.天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193
|
|
| 摘要: |
| [目的]制备抗肿瘤药物藤黄酸（GA）纳米结构脂质载体（GA-NLC），考察其理化性质，并对其抗肿瘤作用进行初步评价。[方法]采用乳化-固化法制备，以粒径、Zeta电位、包封率为评价指标考察其理化性质，并用差示扫描量热法（DSC）进行验证。采用CCK-8法测定GA-NLC对人乳腺癌细胞MDA-MB-231的抑制作用。[结果]制备的GA-NLC粒径分布在20 nm左右，Zeta电位为-（5.86±0.64）mV、包封率为（99.46±0.23）%。DSC结果表明GA以无定型的形式存在于藤黄酸纳米结构脂质载体中。[结论]通过乳化-固化法制备出的GA-NLC，粒径较小、分布均匀，包封率高，与GA溶液相比，GA-NLC具有更强的抗肿瘤活性。 |
| 关键词: 藤黄酸 纳米结构脂质载体 理化性质评价 抑瘤作用 |
| DOI：10.11656/j.issn.1672-1519.2017.01.15 |
| 分类号: |
| 基金项目:天津市自然科学基金（一般项目）（16JCYBJC28200）；国家自然基金（面上项目）（81673605）。 |
|
| The preparation and preliminary evaluation of anti-tumor effect of Gambogic acid nanostructure lipid carrier |
|
HUANG Rui1,2, GUO Pan1,2, LIU Bo-ying1,2, DING Miao-miao1,2, ZHANG Bing1,2, ZHANG Li-na1,2, LIU Zhi-dong1,2
|
|
1.Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Tianjin State Key Laboratory of Modern Chinese Medicine Provice and Ministry Co-established State Key Laboratory Cultivation Base, Tianjn University of Traditional chinese Medicine, Tianjin 300193, China
|
| Abstract: |
| [Objective] To prepare the nanostructure lipid carriers (NLC) incorporated gambogic acid (GA), investigate its physical and chemical properties and evaluate the anti-tumor effect preliminarily.[Methods] Emulsification-evaporation was used to prepare the nanoparticles. The particle size, Zeta potential, encapsulation efficiency were selected as the evaluation indexs to appraise the physical and chemical properties and process validation was implemented by using differential scanning calorimetry (DSC). The CCK-8 assay was used to measure human breast cancer cells MDA-MB-231 cell growth repression rate.[Results] The GA-NLC was about 20 nm in diameter. Zeta potential was -(5.86±0.64) mV. The encapsulation efficiency was (99.46±0.23)%. DSC results showed that GA exists in gambogic acid nanostructure lipid carrier in the form of amorphous.[Conclusion] The GA-NLC prepared by emulsification-evaporation was characterized of smaller particle size, uniform distribution and high encapsulation efficiency. Compared with GA solution, GA-NLC has a significant antitumor activity. |
| Key words: gambogic acid nanostructure lipid carrier physical and chemical properties anti-tumor effect |
