软肝煎对高脂饮食肝纤维化大鼠HA、LN、PCⅢ、CⅣ及病理的影响

Effects of Ruanganjian on the expression of HA、LN、PCⅢ、CⅣ and pathologic changes in hepatic fibrosis rats induced by high fat diet

关键词:  软肝煎  肝纤维化  透明质酸  层连蛋白  Ⅲ型前胶原  Ⅳ型胶原

Key words:  Ruanganjian  liver fibrosis  hyaluronic acid  laminin  procollagen Ⅲ  collagen Ⅳ

摘要:

[目的] 探讨软肝煎对高脂饮食肝纤维化大鼠透明质酸（HA）、层连蛋白（LN）、Ⅲ型前胶原（PCⅢ）、Ⅳ型胶原（CⅣ）及病理的影响。[方法] 高脂饮食确定肝纤维化模型成立后，分别对中药低剂量组和中药高剂量组以每日生药12.8 g/kg体质量和每日生药25.6 g/kg体质量行中药灌胃，模型组、正常组以生理盐水灌胃。治疗12周后处死，观察肝脏组织学改变；取血测血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、甘油三酯（TG）、总胆固醇（TC）；放射免疫法测血清HA、LN、PCⅢ、CⅣ水平。[结果] 两治疗组大鼠血清TC、ALT、AST明显低于模型组；模型组TG明显高于高剂量组。模型组大鼠血清HA、LN、PCⅢ、CⅣ明显高于正常组及两治疗组。肝脏脂肪变性、炎症活动度、肝组织纤维化计分（SSS）结果均显示模型组与中药低剂量组无统计学差异；中药高剂量组低于模型组。[结论] 软肝煎降低肝纤维化大鼠血清ALT、AST、TC、TG、HA、LN、PCⅢ、CⅣ含量，保护肝细胞，改善肝功能，减轻肝脏炎症，抑制胶原合成，促进胶原降解，抑制了肝纤维化的发生、发展。

Abstract:

[Objective] To discuss the effects of Ruanganjian on the expression of HA, LN, PCⅢ, CⅣ and pathologic changes in hepatic fibrosis rats induced by high fat diet.[Methods] After high fat diet liver fibrosis model established, rats in high dose group and low dose group were given intragastric administration by Chinese herb as a dosage of 1.28 g and 2.56 g crude drug for 100 g weight per day, model group and control group were given intragastric administration by saline. Rats were put to death after 12 weeks and the change of liver histology was observed. The level of serum alanine aminotransferase(ALT), aspartate aminotransferase (AST), triglycerides(TG) and total cholesterol(TC) were detected by Hematological examination. The level of serum hyaluronic acid (HA), Laminin (LN), procollagenⅢ(PCⅢ), collagenⅣ(CⅣ) were detected by radioimmunoassay.[Results] The levels of TC, ALT, AST in serum were significantly lower in two treatment groups than those in model group. The levels of TG were higher in model group than those in high dose group. The levels of HA,LN, PCⅢ, CⅣ in serum were higher in model group than those in the rest three groups. The levels of steatosis, steatohepatitis and fibrosis(SSS) was no significant difference between model group and low dose group, and the significance was lower in high dose group than those in model group.[Conclusion] Ruanganjian can decrease the levels of TC, TG, ALT, AST, HA, LN, PCⅢ, CⅣ in serum and protect liver cells, improve liver function, inhibit the production of collagen and improve its degration, to suppress the development of liver fibrosis.