炒蒲黄与蒲黄炭提取物对免疫抑制BALB/c小鼠部分非特异性免疫功能的影响

Effect of part of the non-specific immune function of immune inhibition of BALB/c mice of parched Pollen Typhae and carbonized Pollen Typhae extract

关键词:  BALB/c小鼠  环磷酰胺  免疫抑制  炒蒲黄提取物  蒲黄炭提取物

Key words:  BALB/c male mice  cyclophosphamide  Immune suppression  parched Pollen Typhae extract  carbonized Pollen Typhae extract

摘要:

[目的] 比较炒蒲黄与蒲黄炭提取物对环磷酰胺致BALB/c小鼠免疫功能的影响。[方法] 将正常BALB/c雄性小鼠，随机分为8组，即空白对照组、模型组、炒蒲黄组（生药3.0、6.0、12.0 g/kg）、蒲黄炭组（生药3.0、6.0、12.0 g/kg）。共计灌胃15 d，灌胃第13天开始皮下注射环磷酰胺生理盐水溶液（浓度为每日100 mg/kg）建立免疫抑制动物模型，连续3 d，空白对照组给予同体积生理盐水。通过检测部分免疫器官指数、碳粒廓清实验、血清溶菌酶含量等指标来比较上述药物对免疫抑制BALB/c小鼠非特异性免疫功能强弱。[结果] 炒蒲黄与蒲黄炭均能增加脾脏质量及指数，并且提高单核巨噬细胞系统吞噬功能及溶菌酶活性。[结论] 炒蒲黄与蒲黄炭提取物均可改善环磷酰胺致免疫抑制小鼠的非特异性免疫功能，且蒲黄炭优于炒蒲黄。

Abstract:

[Objective] To compare the effect of parched Pollen Typhae and carbonized Pollen Typhae extract on the immune inhibition of BALB/c mice nonspecific immune function.[Methods] Normal BALB/c male mice were randomly divided into 8 groups, blank control group, model group, parched Pollen typhae(3.0, 6.0, 12.0 g drugs/kg) and carbonized Pollen Typhae(3.0, 6.0, 12.0 g drugs/kg). Intragastric administration for 15 days, 13 days in addition to the blank control group, the rest of the groups subcutaneous inject cyclophosphamide normal saline solution (concentration of 100 mg/kg.d), 3 days in a row, blank group subcutaneous injects equal volume of physiological saline. Through the immune organ index, the indexes of the clearance rate of charcoal particles and the contents of serum lysozyme nonspecific immune index to compare the influence of the drugs on intensity of non-specific immunity function of immune inhibition of BALB/c mice.[Results] Fry cattail pollen and charcoal can enhance the spleen weight and spleen weight index, phagocytosis of mononuclear macrophage system and lysozyme activity.[Conclusion] Parched Pollen Typhae and carbonized Pollen Typhae can strengthen non-specific immune function on the immune inhibition of BALB/c mice, and cattail pollen carbon is better than fried cattail pollen.