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| 炒神曲中短链脂肪酸的HS-GC/MS分析研究 |
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吴丹1,2, 刘双1,2, 谢洪珍3, 杜婕1,2, 杨君君1,2, 王跃飞1,2
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1.天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;2.天津国际生物医药联合研究院, 中药新药研发中心, 天津 300457;3.天津市中药饮片厂有限公司, 天津 300110
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| 摘要: |
| [目的] 建立炒神曲中短链脂肪酸的顶空气相色谱-质谱联用分析方法（HS-GC/MS）。[方法] 采用HS-GC/MS分析炒神曲中短链脂肪酸。炒神曲样品密封于顶空进样瓶中进样分析。[结果] 通过检索美国国家标准与技术研究院（NIST）标准质谱库，鉴定了7种短链脂肪酸：乙酸、丙酸、异丁酸、丁酸、异戊酸、戊酸、己酸。[结论] 该方法能够快速分析炒神曲中短链脂肪酸，为炒神曲的质量控制研究奠定基础。 |
| 关键词: 顶空气相色谱-质谱联用法 炒神曲 短链脂肪酸 |
| DOI：10.11656/j.issn.1672-1519.2017.02.18 |
| 分类号: |
| 基金项目:天津市科技特派员项目（15JCTPJC59100）。 |
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| Analysis of short-chain fatty acids of fried medicated leaven by headspace gas chromatography mass spectrometry |
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WU Dan1,2, LIU Shuang1,2, XIE Hong-zhen3, DU Jie1,2, YANG Jun-jun1,2, WANG Yue-fei1,2
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1.Tianjin State Key Laboratory of Modern Chinese Medicine Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China;3.Chinese Traditional Medicine and Single Chinese Herb, Tianjin 300110, China
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| Abstract: |
| [Objective] To establish a headspace gas chromatography mass spectrometry method for analysis of short-chain fatty acids (SCFAs) of fried medicated leaven. [Methods] Headspace gas chromatography mass spectrometry (HS-GC/MS) was applied for this study. Fried medicated leaven samples were sealed in the headspace bottle for analysis. [Results] By retrieving the National Institute of Standards and Technology (NIST) library, seven SCFAs was identified, including acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid and caproic acid. [Conclusion] The method could be used for rapid analysis of SCFAs of fried medicated leaven, which could establish the foundation for quality control of fried medicated leaven. |
| Key words: headspace gas chromatography mass spectrometry fried medicated leaven short-chain fatty acid |
