基于肿瘤干细胞的西黄丸抗结肠癌作用研究

Antitumor effect of Xihuang pills on colorectal cancer based on cancer stem cells

关键词:  西黄丸  肿瘤干细胞  增殖  迁移  多能干基因  结肠癌

Key words:  Xihuang pills  cancer stem cells  proliferation  migration  multipotent genes  colorectal cancer

摘要:

[目的]探讨西黄丸对结肠癌肿瘤干细胞增殖、迁移及浸润能力的影响并研究其对肿瘤干细胞多能干基因表达的影响。[方法]小鼠CT26结肠癌细胞荷瘤Balb/C小鼠，原代培养肿瘤干细胞，噻唑蓝（MTT）细胞毒性实验分别检测西黄丸对肿瘤干细胞及肿瘤细胞增殖能力的影响；Transwell实验检测西黄丸对肿瘤干细胞浸润及迁移能力的影响；定量逆转录-聚合酶链反应（qRT-PCR）检测西黄丸对肿瘤干细胞多能干基因表达的影响。[结果]西黄丸能有效抑制肿瘤干细胞及肿瘤细胞的增殖，其抑制作用呈剂量依赖性；与肿瘤细胞相比，西黄丸对肿瘤干细胞的直接毒作用更强，其IC50浓度更低；西黄丸还能抑制肿瘤干细胞浸润及迁移，显著下调多能干基因Sox2、Oct4及Nanog的表达。[结论]西黄丸能有效抑制结肠癌肿瘤干细胞的增殖、迁移及浸润，并显著下调多能性基因的表达。西黄丸对肿瘤干细胞的抑制作用可能是其抗结肠癌的基础。

Abstract:

[Objective] To investigate the effect of Xihuang pills on proliferation, invasion and migration abilities of colorectal cancer stem cells(CSCs) and the expression of multipotent genes of colorectal CSCs.[Methods] CT26 induced colorectal cancer cell Balb/C mice, cancer stem and non-stem cells were primarily obtained. The Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was used to determine the proliferation of cancer stem and non-stem cells. The transwell assay was used to determine the effects of Xihuang pills on the invasion and migration abilities of CSCs. qRT-PCR was applied to study the expressions of CSCs multipotent genes after treating cells with Xihuang pills.[Results] Xihuang pills can inhibit the proliferation of CSCs and nCSCs effectively, and the effect is dose-dependent. Xihuang pills have lower IC₅₀ values towards CSCs than nCSCs, which mean it has a more potent direct toxicilty towards CSCs. Further more, it can inhibit the invasion and migration of CSCs, and down-regulate the expression of multipotent genes such as Sox2, Oct4 and Nanog significantly.[Conclusion] Xihuang pills can inhibit the proliferation, invasion and migration abilities of colorectal CSCs effectively, and down-regulate the expression of multipotent genes dramatically. The anti-colorectal effect of Xihuang pills may contribute to its inhibition of CSCs.