佩兰对2型糖尿病合并脂代谢紊乱大鼠肝脏肌醇必需酶1α表达的影响

Influence of the inositol-requiring enzyme 1a(IRE1α) in the liver getted mice with type 2 diabetes mellitus and hyperlipidemia after treatment by the fortune's eupatoium herb

关键词:  2型糖尿病  脂代谢紊乱  佩兰  IRE1α

Key words:  fortune's eupatorium herb  nositol-requiring enzyme 1α  type 2 diabetes mellitus  hyperlipidemia

摘要:

[目的]研究佩兰中药颗粒对2型糖尿病合并脂代谢紊乱大鼠肝脏肌醇必需酶1α（IRE1α）表达的影响。[方法]将造模成功的合并脂代谢紊乱的2型糖尿病大鼠按随机数字表法随机分为模型组、佩兰中药组、吡格列酮组，每组9只，另设空白对照组10只。佩兰中药组予佩兰中药颗粒按3 g/（kg·d）灌胃给药，吡格列酮组0.3 mg/（kg·d）灌胃，模型组及空白对照组予生理盐水灌胃，持续给药5周，检测各组大鼠血糖、血清甘油三酯（TG）、血清总胆固醇（TC）、血清游离脂肪酸（FFA）水平，检测肝脏IRE1α基因及蛋白表达水平。[结果]经治疗后，佩兰中药组大鼠的血清TG、TC水平明显低于模型组，肝脏IRE1α的基因及蛋白表达水平明显高于模型组。[结论]佩兰可以提高2型糖病合并脂代谢紊乱大鼠肝脏中IRE1α的表达。

Abstract:

[Objective] To reserch the influence of inositol-requiring enzyme 1α(IRE1α) in liver getted mice with type 2 diabetes mellitus and hyperlipidemia after treatment by fortune's eupatorium herb.[Methods] The successful builted mice were randomly assigned into four groups, model group feed by 2 mL/d saline, peilan treatment group feed by fortune's eupatorium herb 3 g/(kg·d) and pioglitazone treatment group feed by pioglitazone in 0.3 mg/(kg·d), and 10 normal mice were sized to be a group. Mice were treated for 5 weeks, and the blood glucose, triglyceride, cholestenone and insulin were invested, then the expression of the IRE1α's mRNA and protein were invested.[Results] Compared with the model group,the triglyceride and cholestenone in fortune's eupatorium herb treatment group turned to be better, and the impression of mRNA and protein in liver was lower.[Conclusion] Fortune's eupatorium herb can improve the content of IRE1α in the organs of mice with type 2 diabetes mellitus and hyperlipidemia.