HPLC方法同时测定独活中二氢欧山芹醇-β-D葡萄糖苷和二氢欧山芹醇的含量

Simultaneous determination of columbianetin-β-D-glucopyranoside and columbianetin in Angelicae pubescentis radix by HPLC method

关键词:  独活  二氢欧山芹醇-β-D葡萄糖苷  二氢欧山芹醇  高效液相色谱法

Key words:  Angelicae pubescentis radix  columbianetin-β-D-glucopyranoside  columbianetin  HPLC

摘要:

[目的]研究独活中二氢欧山芹醇-β-D葡萄糖苷和二氢欧山芹醇的最佳提取条件，同时建立了同时测定独活中二氢欧山芹醇-β-D葡萄糖苷和二氢欧山芹醇含量的高效液相色谱法（HPLC）。[方法]通过单因素分析法考察了提取溶剂、料液比、超声提取时间对独活提取物中二氢欧山芹醇-β-D-葡萄糖苷和二氢欧山芹醇含量的影响。采用HPLC法对不同提取条件和不同产地的独活中两种成分含量。[结果]独活中二氢欧山芹醇-β-D葡萄糖苷和二氢欧山芹醇的最佳提取条件为提取溶剂用100%的甲醇，料液比为1∶50，超声提取60min。二氢欧山芹醇-β-D-葡萄糖苷在0.3125~20 μg/mL内呈良好线性，平均回收率为99.0%（RSD=2.99%）；二氢欧山芹醇在0.3125~20 μg/mL内呈良好线性，平均回收率为102%（RSD=2.59%）。[结论]所建立的高效液相色谱法，能够准确测定独活中二氢欧山芹醇-β-D葡萄糖苷和二氢欧山芹醇含量，可用于独活药材的质量评价。

Abstract:

[Objective] To research the best extraction process about columbianetin-β-D-glucopyranoside and columbianetin in Angelicae pubescentis radix and develop a simple, accurate, repeatable method for simultaneous determination of columbianetin-β-D-glucopyranoside and columbianetin in Angelicae pubescentis radix by High Performance Liquid Chromatography(HPLC).[Methods] The sample was extracted by different extraction solvent, extraction time, ratio of liquid to material to analysis the effect about the content of columbianetin-β-D-glucopyranoside and columbianetin.[Results] The optimum level were methanol, 60 min, 1:50, respectively. Two calibration curves exhibited good linearity (r=1) within the linear range from 0.312 5 μg/mL to 20 μg/mL. The average percentages of recovery was 99.0% and 102%, and the RSD was 2.99% and 2.59%, respectively.[Conclusion] The developed simple HPLC method could successfully be utilized to determine two components in samples from different regions. It was concluded that this new method could determine columbianetin-β-D-glucopyranoside and columbianetin for quality control of Angelicae pubescentis radix.