| 摘要: |
| [目的]探讨补肾调冲方对多囊卵巢综合征患者子宫内膜的保护作用。[方法]诊刮获取多囊卵巢综合征患者的子宫内膜组织，体外混合培养子宫内膜细胞，分别予以补肾调冲方、孕酮、17β-雌二醇干预子宫内膜细胞，并设置空白对照组。利用透射电镜观察子宫内膜细胞超微结构；ELISA法检测子宫内膜细胞中孕激素受体的表达，逆转录-聚合酶链反应（RT-PCR）及Western blot检测转化生长因子（TGF-β1）、Smad4 mRNA及蛋白的表达。[结果]药物干预后经透射电镜观察发现，与雌激素组、空白对照组相比，实验组、孕激素组细胞表面微绒毛数量增多，有分支，线粒体体积增大，粗面内质网扩张，高尔基体肿胀；实验组子宫内膜细胞中孕激素受体表达低于雌激素组、空白对照组，差异有统计学意义（P<0.05）；与雌激素组、空白对照组相比，实验组TGF-β1、Smad4 mRNA及蛋白表达增多，差异有统计学意义（P<0.05）.[结论]补肾调冲方能够促进多囊卵巢综合征患者子宫内膜由增生期向分泌期转变。 |
| 关键词: 多囊卵巢综合征 子宫内膜 补肾调冲方 孕激素受体 TGF-β1/Smad4 |
| DOI：10.11656/j.issn.1672-1519.2017.06.14 |
| 分类号:R711.75 |
| 基金项目:天津市中医中西医结合科研项目（13097）. |
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| Study on the effects of endometrium of women with polycystic ovary syndrome by Bushen Tiaochong decoction |
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GUO Jie1, WANG Yue-ping2, ZHANG Wei1, DU Yong-hong1, WANG Yu-hua1, SONG Dian-rong1
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1.The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China;2.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] Study on the effects of Bushen Tiaochong decoction on endometrium of women with polycystic ovary syndrome. [Methods] In vitro cultivation human endometrium cells of women with polycystic ovary syndrome. Endometrial cells were be divided into the experimental group treated with Bushen Tiaochong decoction, positive control group treated with progesterone, negative control group treated with 17 beta estradiol and the blank control group. Observation the ultrastructure changes of endometrium cells after drugs intervention by transmission electron microscope. Assay the expression of progesterone receptor in endometrial cells for each group by ELISA. Detect the expression of TGF-β1 and Smad4's mRNA by semi-quantitative RT - PCR and the expression of TGF-β1 and Smad4's protein by Western blot. [Results] Compared with the estrogen group and the control group, the quantity of microvilli on cells' surface and the volume of mitochondria of the progesterone group and the experimental group increased. The rough endoplasmic reticulum and the Golgi apparatus of the progesterone group and the experimental group swelled. The concentration of progesterone receptor in endometrial cells of experimental group was lower than that of the estrogen group and the control group (P<0.05). [Conclusion] Bushen Tiaochong decoction can promote the transformation of endometrium from proliferative phase to secretive phase. |
| Key words: PCOS endometrium Bushen Tiaochong decoction PR TGF-β1/Smad4 |
