利用清热明目方超声雾化法干预兔眼结膜炎模型的实验研究

Experimental research on conjunctivitis treated with Qingre Mingmu decoction by ultrasonic atomization method

关键词:  清热明目方  超声雾化法  结膜炎

Key words:  Qingre Mingmu decoction  ultrasonic atomization  conjunctivitis

摘要:

[目的]探讨利用清热明目方超声雾化法对兔眼结膜炎模型的治疗作用及机制研究。[方法]采用清热明目方水煎剂作为实验样品，以左氧氟沙星滴眼液作对照，用斑蝥酊刺激兔眼造成结膜炎模型，观察各实验组动物的眼刺激症状、结膜组织病理学变化及免疫组织化学方法观察白介素-1β（IL-1β）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）在兔眼结膜的表达情况。[结果]各组确定造模成功即刻进行相应的治疗。病理组织学观察清热明目组、阳性对照组与模型组比较有统计学差异（P<0.05）.IL-1β阳性对照组与清热明目组在上皮细胞层表达较少，均优于模型组。IL-6、TNF-α模型组上皮层表达最明显。[结论]清热明目方超声雾化法治疗结膜炎有明显的疗效。对相关炎性因子IL-1β、IL-6 和TNF-α进行免疫组织化学定位，进行定性分析，表明结膜炎与其有密切的关系。结膜上皮炎性因子的表达可以反映疾病的严重程度。

Abstract:

[Objective] To take the study on efficacy and the mechanism of the conjunctivitis model with Qingre Mingmu decoction by ultrasonic atomizing method.[Methods] Put Qingre Mingmu decoction as test samples, and the left Ofloxacin Eye Drops as control. Establish rabbit conjunctivitis model with micro-injector trickle of cantharid in tincture. Then observe the eye irritation symptom, conjunctiva histopathology and the expression of IL-1beta, IL-6, TNF-alpha by immunohistochemical staining of every group. [Results] Each group determine the successful model and immediately corresponding treatment. Comparing with the model group, pathological histological observation of Qingre Mingmu concentration group and the control group had significant difference (P<0.05). IL-1beta control group and traditional Chinese medicine concentrations less expression in the epithelial cell layer, but both better than the model group.IL-6 and TNF-alpha were mainly expressed in the epithelial cell layer, Model group on the cortical expression of the most obvious difference. [Conclusion] There was obvious effect on Qingre Mingmu ultrasonic atomization method treating conjunctivitis. Immunohistochemical localization of rabbit conjunctivitis model of inflammatory factors of IL-1 β, IL-6 and TNF- α, and qualitative analysis, show that there is a close relationship between conjunctivitis and the factors. The severity of inflammatory factor expression can reflect the conjunctival disease.