补阳还五汤对高血压前期大鼠T淋巴细胞亚群的影响

Effect of Buyang Huanwu decoction on the T lymphocyte subsets of prehypertension rats

关键词:  补阳还五汤  盐敏感大鼠  高血压前期  T淋巴细胞亚群比例

Key words:  Buyang Huanwu decoction  salt sensitive rat  prehypertension  the percent of T lymphocyte subsets

摘要:

[目的]通过观察补阳还五汤对高盐所致高血压前期大鼠T淋巴细胞亚群比例及γ-干扰素（INF-γ）、肿瘤坏死因子-α（TNF-α）的影响，探讨补阳还五汤在调节高血压前期T细胞免疫中的作用机制。[方法]选取7周龄盐敏感大鼠（DS）与盐抵抗大鼠（SS）各20只，SS大鼠随机分为正常组和正常给药组，DS大鼠随机分为模型组和治疗组。各组大鼠适应性喂养后，改用高盐饲料喂养，同时监测大鼠尾动脉收缩压，待其血压值升高至120~139/80~89 mmHg （1 mmHg=0.133 kPa）这一范围时，确定为高血压前期大鼠模型，并以补阳还五汤灌胃治疗。治疗结束后大鼠腹主动脉取血，以流式细胞术检测全血中T淋巴细胞CD4⁺与CD8⁺的百分率，并计算CD4⁺/CD8⁺的比值。采用酶联免疫吸附测定（ELISA）方法检测血清中INF-γ、TNF-α的含量。[结果]补阳还五汤可以下调CD4⁺T淋巴细胞及CD8⁺T淋巴细胞百分率，上调CD4⁺/CD8⁺的比值，降低TNF-α、INF-γ的含量。[结论]补阳还五汤可能是通过下调CD4⁺T淋巴细胞与CD8⁺T淋巴细胞的百分率以及INF-γ、TNF-α细胞因子的表达，改善高血压前期机体T细胞免疫功能及其炎症反应，延缓高血压前期的发展进程。

Abstract:

[Objective] To investigate the effect of Buyang Huanwu decoction on the T lymphocyte subsets of prehypertension rat and expression of associated cell factor INF-γ, TNF-α.[Methods] The 20 male salt sensitive rats (DS) of 7 weeks were selected and randomly divided into normal group and normal administration group. The 20 male salt resistant rats(SS) of 7 weeks were selected and randomly divided into model group and treatment group. After one week of adaptive feeding, rats were fed by 8% high-salt feed began to make the model, systolic arterial pressure(SAP) of tail was detected at the same time. When the SAP was at the range of 120~139/80~89 mmHg (1 mmHg=0.133 kPa), the model was successfully established, and rats in treatment group and normal administration group treated with Buyang Huanwu decoction gavage. Abdominal aortic blood of all the rats was taken after the end of the treatment. Flow cytometer was used to the percent of CD4⁺ T lymphocytes and CD8⁺ T lymphocytes, and the ratio of CD4⁺/CD8⁺ was calculated. ELISA method was used to detect the content of INF-γ and TNF-α in serum.[Results] Buyang Huanwu decoction can down the percent of CD4⁺ T lymphocytes and CD8⁺ T lymphocytes, increase the ratio of CD4⁺/CD8⁺ and decrease the contents of INF-γ and TNF-α.[Conclusion] Buyang Huanwu decoction can improve the body's immune function and inflammation to delay the progress of prehypertension by decreasing the percent of CD4⁺ T lymphocytes and CD8⁺ T lymphocytes and the expression of associated cell factor INF-γ, TNF-α probably.