苏合香早期干预促进大鼠脑缺血后长期神经功能修复

Early intervention of storax promotes long-term neurological function restoration after ischemic stroke in rats

关键词:  苏合香  脑缺血再灌注  神经功能修复  神经血管重塑

Key words:  storax  cerebral ischemia-reperfusion  neuronal function restoration  neurovascular remolding

摘要:

[目的]研究苏合香早期干预对大鼠脑缺血后长期神经功能修复的影响。[方法]将健康雄性SD大鼠随机分为对照组和苏合香组，线栓法建立大脑中动脉阻塞（MCAO）模型，于脑缺血后1、3、6 h各予苏合香灌胃给药1次。于脑缺血后第1、4、7、14、21、28 d时，采用改良的神经功能缺损评分（mNSS）、胶带去除实验、足误实验以测试大鼠神经功能，并记录上述各时点大鼠体质量及死亡率。采用苏木精-伊红（HE）染色以观察脑组织病理修复，采用免疫荧光技术以观察缺血周边脑组织中血小板-内皮细胞粘附分子（CD31）、突触小泡蛋白（SYP）的表达。[结果]大鼠体质量在造模前均一，在脑缺血1、4 d时下降、缺血7~28 d时逐渐上升，相比于对照组，苏合香组体质量降幅较小而增幅较大，但两组间各时间点同比无显著差异；大鼠mNSS评分在缺血1 d时较高，缺血7、14、21、28 d时逐渐降低，苏合香组各时间点mNSS评分均低于对照组，其中缺血21、28 d时的mNSS评分较对照组显著降低（P<0.05）；缺血1 d时胶带去除时间及行走中患肢足误步数明显升高，后逐渐降低，同比于对照组，苏合香组各时点胶带去除时间及肢体足误步数均下降，并显著降低了脑缺血4、7、14 d时肢体足误步数（P<0.05）。HE染色结果显示苏合香组脑组织修复程度优于对照组，同时苏合香组缺血周边脑组织中的CD31、SYP阳性表达量较对照组显著增加（P<0.05）。[结论]苏合香早期干预可促进脑缺血后长期神经功能恢复及内源性神经血管重塑。

Abstract:

[Objective] To investigate whether early intervention of storax promotes long-term neurological function restoration after ischemic stroke in rats.[Methods] Healthy male SD rats were randomly divided into vehicle and storax groups. Rats were subjected to 2 hours of occlusion of middle cerebral artery (MCAO), then administered with storax intragastrically at 1, 3, 6 hours after MCAO for 3 times. Modified neurological severity scores (mNSS), adhesive tap-removal test and foot fault test were performed at 1, 4, 7, 14, 28 days after MCAO to evaluate neurological function, at the same time, weight and death rate were recorded. Hematoxylin eosin (HE) staining was performed to observe repairmen of brain tissue, and immunofluorescence staining of platelet endothelial cell adhesion molecule-1 (CD31) and synaptophysin (SYP) was also performed.[Results] The weight of rats were decreased at 1, 4 days after MCAO and gradually increased from 7 days to 28 days after MCAO, compared vehicle group. Weight reduction was smaller and weight incretion was bigger in storax group, but there was no significant difference between the two groups. The mNSS scores, the tap-removal time and the foot-fault steps were increased at 1 day and gradually decreased till 28 days after MCAO, storax decreased the mNSS scores, the tap-removal time and the foot-fault steps at the above time points, and significantly decreased the mNSS scores at 21, 28 days and foot-fault steps at 4, 7, 14 days after MCAO (P<0.05). HE staining showed that brain tissue in storax group got a better repairment than that of vehicle group. Immunofluorescence staining showed that CD31 and SYP positive expression in ischemic border were significantly increased in storax group (P<0.05) when compared with vehicle group.[Conclusion] Early intervention of storax promotes long-term neurological function restoration and neurovascular remolding after stroke in rats.