五田保肝液对酒精性肝病大鼠肝组织Fas、Fasl、Caspase-8蛋白表达的影响

Effects on protein expression of Fas, Fasl and Caspase-8 in alcohol liver disease of rats intervented by Wutian Baogan Ye

关键词:  五田保肝液  酒精性肝病  Fas  Fasl  Caspase-8

Key words:  Wutian Baogan Ye  alcohol liver disease  Fas  Fasl  Caspase-8

摘要:

[目的]研究五田保肝液对酒精性肝病大鼠肝细胞凋亡以及肝组织中Fas、Fasl、Caspase-8蛋白表达的影响。[方法] 100只Wistar雄性大鼠随机分为正常对照组10只，阳性药对照组、模型组、五田保肝液低、中、高剂量组各18只。采用白酒灌胃的方法制备动物模型，造模12周乙醚麻醉后断头处死大鼠，取肝组织，采用TUNEL法检测肝细胞凋亡指数，采用免疫组化法法检测肝组织Fas、Fasl、Caspase-8蛋白的表达。[结果]模型组凋亡指数高于其他各组，中药中、高剂量组和阳性药对照组凋亡指数低于模型组，中药低剂量组凋亡指数和模型组无显著性差异；各组Fas蛋白表达差异没有显著性。模型组Fasl、Caspase-8蛋白表达高于其它各组；五田保肝液中、高剂量和阳性药组大鼠肝组织Fasl、Caspase-8的表达较模型组降低，五田保肝液低剂量组Fasl的表达与模型组相比，虽然从数据趋势上有下降的趋势，但差异无统计学意义。五田保肝液低剂量组Caspase-8的表达和模型组差异无统计学意义。[结论]五田保肝液可能通过下调Fasl、Caspase-8的表达，减少酒精引起的大鼠肝细胞的凋亡，从而达到防治酒精性肝病的功效。

Abstract:

[Objective] To investigate the Wutian Baogan Ye's effect on the apoptosis of liver cells, and the expression of Fas, Fasl, Caspase-8 in liver tissue of alcohol liver disease (ALD) rats.[Methods] The 100 Wistar rats were divided into six groups randomly:normal group (10), model control group(18),Wutian Baogan Ye low dose group(18), middle dose group(18), high dose group(18), positive drug group(18). Rat model of ALD was induced by perfusing stomach with liquor. After 12 weeks, rats were anesthesia by diethyl ether and decapitation to execute. Hepatic tissue of rats was superscription. Index of apoptosis was detected by TUNEL. Expression of Fas, Fasl in liver tissue of rats were observed by immunohistochemisty.[Results] Compard with normal rats, the index of apoptosis was higher in model group, and Wutian Baogan Ye group (middle and high dose) and positive drug group was lower than that in the model group. There was no significant difference between low dose group and model group in the index of apoptosis. Expression of Fas was not different among groups. The expression of Fasl and Caspase-8 in model group was significant higher than other groups. Compared with model rats, Wutian Bangan Ye (middle and high dose) groups and positive drug can decreased the expression of Fasl and Caspase-8. Wutian Baogan Ye low dose group decreased the erpression of Fasl but had no statistical significance compared with model group.[Conclusion] Wutian Baogan Ye had an active effect on inhibiting apoptosis of liver cell in ALD through decreased the expression of Fasl, Caspase-8 to treat and protect from ALD.