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| 异槲皮苷对db/db小鼠肝脏脂肪酸代谢及相关基因表达的影响 |
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许光远1, 孙文2, 宋紫临2, 郭璇2, 吴丽丽2, 秦灵灵2, 侯丹3, 张茁3, 田硕3, 李响4, 刘铜华2
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1.首都医科大学附属复兴医院中医科, 北京 100045;2.北京中医药大学中医养生学教育部重点实验室, 北京 100029;3.陕西中医药大学第一附属医院老年病科, 咸阳 712046;4.河北工程大学医学院, 邯郸 056000
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| 摘要: |
| [目的] 研究异槲皮苷对自发性糖尿病小鼠肝脏脂肪酸代谢相关基因表达的调控作用。[方法] 8周龄雄性db/db小鼠随机分为模型组、异槲皮苷组，雄性C57BL/6J小鼠为正常对照组，药物干预4周后，检测小鼠体质量、空腹血糖（FBG）、总胆固醇（CHO）、甘油三酯（TG）、游离脂肪酸（FFA），肝质量和肝质量/体质量；肝组织苏木精-伊红（HE）染色观察肝细胞脂质蓄积；逆转录聚合酶链式反应（RT-PCR）基因表达。[结果] 与模型组比较，异槲皮苷组小鼠体质量、FBG、CHO、TG、FFA、肝质量、肝质量/体质量显著降低（P<0.05或P<0.01）；苏木精-伊红（HE）染色肝细胞脂肪变性减轻，肝脏SREBP1c、FAS mRNA表达降低（P<0.05；P<0.01），PGC1α、PPARαmRNA表达显著升高（P<0.05）。[结论] 异槲皮苷可以改善db/db小鼠肝脏脂肪酸代谢紊乱，可能是通过下调肝脏SREBP1c、FASmRNA表达，上调肝脏PGC1α、PPARα表达实现的。 |
| 关键词: 异槲皮苷 脂肪酸代谢 基因表达 |
| DOI：10.11656/j.issn.1672-1519.2017.11.17 |
| 分类号:R285.5 |
| 基金项目:北京市教育委员会高校重大成果转化项目。 |
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| Improvement of Isoquercitrin on liver fatty acid metabolism-related gene expression in db/db mice |
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XU Guang-yuan1, SUN Wen2, SONG Zi-lin2, GUO Xuan2, WU Li-li2, QIN Ling-ling2, HOU Dan3, ZHANG Zhuo3, TIAN Shuo3, LI Xiang4, LIU Tong-hua2
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1.Department of Endocrinology, Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100029, China;2.Institute of Health cultivation, Beijing University of Chinese Medicine, Beijing 100029, China;3.Department of Geriatrics, The First Affiliated Hospital of Shaanxi University of traditional Chinese Medicine, Xianyang 712000, China;4.Medical College of Hebei University of Engineering, Handan 056000, China
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| Abstract: |
| [Objective] To investigate the effects and mechanisms of Isoquercitrin on liver fatty acid metabolism.[Methods] The 8 week old male db/db mice were randomly divided into two groups, control group and Isoquercitrin group. The 6 male C57BL/6J mice were used as normal group. Examinations were performed, including body weight, serum glucose, lipid, histopathology and expression of mRNA after a 4-weektreatment period.[Results] Compared with the Control group, Isoquercitrin group body weight, FBG, CHO, TG, FFA, liver weight, liver weight/body weight of mice decreased significantly (P<0.05 or P<0.01), and improved fat deposition of liver tissue by histological examination. The expression of SREBP1c, FAS mRNA in liver of Isoquercitrin group decreased (P<0.05 or P<0.01), PGC1α, PPARα mRNA expression was significantly increased (P<0.05).[Conclusion] The effect of Isoquercitrin improving the db/db mice fatty acid metabolism may be by down-regulating the expression of SREBP1c, FAS mRNA, and up-regulating the expression of PGC1α, PPARα mRNA. |
| Key words: Isoquercitrin fatty acid metabolism gene expression |
