白芍总苷对滑膜成纤维细胞增殖、凋亡及周期影响的实验研究

Experimental research on the influence of total glucosides of paeonia on the proliferation, apoptosis and cell cycle of fibroblast-like synovial cells

关键词:  白芍总苷  骨关节炎  滑膜炎症  异常增殖

Key words:  total glucosides of paeonia  osteoarthritis  synovial inflammation  abnormal growth

摘要:

[目的] 探讨传统中药白芍的主要成分白芍总苷（TGP）对滑膜成纤维细胞（FLS）生长的调控机制。[方法] 以FLS为作用对象，噻唑兰（MTT）比色法对不同浓度TGP干预下的FLS增殖情况进行检测。流式细胞术（FCM）对不同浓度TGP干预下的FLS凋亡和周期情况进行检测。[结果] TGP对FLS活力的调控呈显著的浓度依赖性，主要通过诱导FLS晚期凋亡（P<0.05）、延长细胞周期中的G2期（P<0.05）并同时阻滞S期（P<0.05）来抑制骨关节炎（OA）滑膜炎症中FLS的过度增殖。[结论] TGP可通过调节FLS的增殖、凋亡和周期，抑制FLS的异常生长，这个研究结果也为中药活性成分在滑膜炎症中的调控机制和疗效肯定奠定了基础。

Abstract:

[Objective] To discuss the regulatory mechanism of total glucosides of paeonia (TGP) on proliferation, apoptosis and cell cycle of fibroblast-like synovial cells (FLS) in the process of osteoarthritis (OA).[Methods] With FLS being the object of action, Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was used to detect the proliferation of FLS, and flow cytometry (FCM) was used to detect the apoptosis and cell cycle.[Results] The concentration dependence of FLS was existed in the concentration of TGP. In addition, the inhibitory effect of TGP on FLS mainly through inducing late apoptosis, as well as prolonging G2 phase (P<0.05) and blocking S phase (P<0.05) in the cell cycle of FLS.[Conclusion] TGP could inhibit the abnormal growth of FLS by regulating its proliferation, apoptosis and cell cycle. The findings above laid the groundwork for confirming the regulatory mechanism and curative effect of active components of Chinese medicine.