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| 哈氏蜈蚣线粒体基因组全序列分析 |
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孙丽冉1,2, 齐迎菊1,2, 田晓轩1,2
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1.天津中医药大学, 天津市现代中药重点实验室, 天津 300193;2.天津国际生物医药联合研究院中药新药研发中心, 天津 300457
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| 摘要: |
| [目的] 获取哈氏蜈蚣线粒体基因组全序列。[方法] 应用差速离心以及滚环扩增的方法得到纯度较高的线粒体DNA，采用Illumina HiSeq X Ten技术对扩增得到的哈氏蜈蚣线粒体DNA进行测序，采用从头组装和mapping近缘种的方法拼接组装线粒体基因组，应用MITOS在线注释及与近缘种进行本地注释等方法注释线粒体基因组。[结果] 获得14 538 bp哈氏蜈蚣线粒体基因组全序列。其共有20个tRNA及13个蛋白基因，2个rRNA（16S rRNA、12S rRNA）。[结论] 获取哈氏蜈蚣线粒体基因组全序列，并可用于药用蜈蚣的资源调查与保护研究。 |
| 关键词: 哈氏蜈蚣 线粒体基因组 线粒体DNA 序列分析 |
| DOI：10.11656/j.issn.1672-1519.2018.03.19 |
| 分类号:R284.2 |
| 基金项目:横向课题：疏血通注射液基原研究（HX2017-18）。 |
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| Analysis of mitochondrial genome of Scolopendra subspinipes dehaani |
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SUN Liran1,2, QI Yingju1,2, TIAN Xiaoxuan1,2
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1.Tianjin Key Laboratory of Modern Chinese Medcine, Tianjin University of Traditional Chinese Medcine, Tianjin 300193, China;2.Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biomedicine, Tianjin 300457, China
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| Abstract: |
| [Objective] To investigate the complete mitochondrial genome of Scolopendra subspinipes dehaani.[Methods] Mitochondrial DNA of Scolopendra subspinipes dehaani was purified by differential centrifugation and rolling circle amplification. The Illumina Hiseq X Ten technique was chosen to sequence the purified mitochondrial DNA, and then the strategy of de novo assembly and mapping versu mtgenome of closely related species by MIRA 4.0 was adopted to get the complete mitochondrial sequence. This genome was annotated by Online Server MITOS and mtgenome of closely related species.[Results] The 14 538 bp complete mitochondrial genome of Scolopendra subspinipes dehaani with high coverage contains 20 transfer RNA,13 protein coding genes, 2 ribosomal RNA genes (16S rRNA, RS rRNA).[Conclusion] The mtgenome of Scolopendra subspinipes dehaani was obtained, and could be further applied in the resources survey and protection of medical centipede. |
| Key words: Scolopendra subspinipes dehaani mitochondrial genome mitochondrial DNA sequence analysis |
