七子益肾理冲汤对DOR大鼠卵巢储备的影响

Effect of Qizi Yishen Lichong decoction on ovarian reserve in rats model

关键词:  卵巢储备功能  抗穆勒管激素  不孕  七子益肾理冲汤  肖承悰

Key words:  ovarian reserve  anti-mullerian hormone  infertility  Qizi Yishen Lichong decoction  XIAO Chengcong

摘要:

[目的]探讨七子益肾理冲汤对脱氧乙烯基环己烯（VCD）致卵巢储备功能低下（DOR）大鼠卵巢储备功能的影响。[方法]60只雌性SD大鼠随机分为空白组、模型组、中药低、中、高剂量组及脱氢表雄酮（DHEA）组。模型组采用VCD注射制作DOR大鼠模型。观察卵巢指数，HE染色观察卵巢组织形态，放射免疫法检测促卵泡刺激素（FSH）、血清雌二醇（E₂）及抗穆勒管激素（AMH）水平。[结果]与空白组比较，模型组卵巢组织萎缩，各级卵泡数目减少，卵巢指数和E₂、AMH水平降低，FSH水平升高（P<0.05）；与模型组比较，中药高剂量组卵巢指数上升，卵巢组织形态明显改善，原始卵泡和黄体增加，闭锁卵泡减少；E₂、AMH水平升高，FSH水平降低（P<0.05）。[结论]七子益肾理冲汤可修复卵巢储备的损失，促进卵泡生长，抑制卵泡闭锁。

Abstract:

[Objective] To investigate the effect of Qizi Yishen Lichong decoction on ovarian reserve in DOR rats model.[Methods] The 60 female SD rats were divided into six groups:blank group, model group, CM low-dose group, CM normal-dose group, CM high-dose group and DHEA group. The ovarian index was calculated, the ovarian HE slides were observed under microscope, and blood FSH, E₂ and AMH levels were tested by Elisa.[Results] Compared with blank group, model group had shown ovarian tissue atrophy in HE slides, numbers of all types of ovarian follicles declined, FSH level increased significantly, while ovarian index, E₂ and AMH levels significantly lowered (P<0.05); compared with model group, CM high-dose group has shown a better ovarian appearance in HE slides, increased in numbers of ovarian primordial follicles and corpus luteum, decreased in numbers of atresia follicles, ovarian index, E₂ and AMH levels significantly increased (P<0.05), and significantly drop of FSH level (P<0.05).[Conclusion] Qizi Yishen Lichong decoction might repair the loss of ovarian reserve, stimulate the growth of ovarian follicles and reduce ovarian follicle apoptosis.