| 摘要: |
| [目的]研究补骨脂苷（PO）和异补骨脂苷（IPO）与牛血清白蛋白（BSA）的相互作用。[方法]通过紫外吸收光谱法、荧光淬灭光谱法、同步荧光光谱法、三维荧光光谱法分析PO和IPO对牛血清白蛋白的淬灭机制、结合常数、结合位点数及构象影响。[结果]PO和IPO对牛血清白蛋白产生了静态淬灭作用，两种药物与BSA有较弱的结合，在BSA上的结合位点均为位点Ⅱ，并且对BSA上色氨酸氨基酸残（Trp）基微环境产生了微弱的影响。[结论]借助光谱法揭示了PO和IPO与BSA相互作用的机制。 |
| 关键词: 补骨脂苷 异补骨脂苷 紫外吸收光谱法 荧光光谱法 |
| DOI：10.11656/j.issn.1672-1519.2018.06.16 |
| 分类号:R285.5 |
| 基金项目:天津市科技创新体系及条件平台建设项目（14TXZYJC00440）；天津市应用基础与前沿技术研究计划一般项目（15JCYBJC29300）。 |
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| Study on the interaction between BSA and psoralenoside as well as isopsoralenoside |
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GUO Jiading1, HAO Jia1, LI Mengrong1, WANG Yucheng1, LIU Erwei2
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1.Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin 300193, China
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| Abstract: |
| [Objective] To study of on the interaction between BSA and psoralenoside (PO) as well as isopsoralenoside (IPO).[Methods] Using the methods of UV absorption spectroscopy, fluorescence quenching spectroscopy, synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy quenching mechanism, binding constants, binding sites, conformational changes of PO as well as IPO to the BSA were analyzed.[Results] PO and IPO bound weakly to BSA with a static quenching process, which cause light changes in the polarity environment around the chromophore of tryptophan. The binding sites of the two drugs on BSA were Site Ⅱ.[Conclusion] The mechanism of interaction between PO and IPO with BSA was revealed through the spectroscopy study. |
| Key words: psoralenoside isopsoralenoside UV absorption spectroscopy study fluorescence spectroscopy study |
