补骨脂果皮和种子中化学成分的分布规律研究

Distribution rule of compounds in pericarp and seed of Fructus Psoralea

关键词:  生补骨脂  果皮  种子  超高效液相色谱法

Key words:  Fructus Psoralea  pericarp  seed  UPLC

摘要:

[目的]阐明豆科植物补骨脂果皮、种子中化学成分的分布规律。[方法]采用超高效液相色谱法测定四批生补骨脂果皮、种子中香豆素、黄酮、单萜等化合物，计算各化学成分在补骨脂果实中的相对含量。[结果]补骨脂苷、异补骨脂苷主要分布在补骨脂种子中，其相对含量≥ 89.63%；补骨脂甲素、新补骨脂异黄酮、Corylifol A、Corylifol C、补骨脂查尔酮、补骨脂酚等黄酮和单萜酚类成分主要分布在果皮中，其相对含量≥ 80.86%，其中Corylifol C仅分布于果皮中；补骨脂素、异补骨脂素、补骨脂定、4″，5″-去氢异补骨脂定等香豆素类成分在种子和果皮中均有分布，其相对含量相当。[结论]补骨脂苷和异补骨脂苷主要存在于补骨脂种子中，黄酮和单萜类化合物主要存在于果皮中，补骨脂素、异补骨脂素等脂溶性香豆素在果皮和种子中均有分布。

Abstract:

[Objective] To illuminate the distribution rule of compounds in pericarp and seed of Fructus Psoralea.[Methods] Ultra performance liquid chromatography equipped with diode array detector was employed to simultaneously determine the compounds (coumarin, flavone and monoterpene phenol) in pericarp and seed of Fructus Psoraleas.[Results] Psoralenoside and isopsoralenside were mainly distributed in seed with relative content ≥ 89.63%. Flavone and monoterpene phenol were mainly distributed in the pericarp, and the relative content of that was more than 80.86%, and corylifol C was only distributed in the pericarp. However, the coumarins such as psoralen, isopsoealen, psoralidin and 4″, 5″-dehydroisopsoralidin were distributed both in seed and pericarp, and the relative content of which was similar.[Conclusion] Psoralenoside and isopsoralenoside were found to mainly exist in seed of Fructus Psoralea. A large proportion of flavonoids and monoterpene phenolic compound were present in the pericarp of Fructus Psoralea. Coumarins with fat-solubility, such as psoralen and isopsoralen, were found in the pericarp and seed of Fructus Psoralea.