通腑泻肺法对ALI/ARDS大鼠肠屏障的保护作用

Protective effect of Tongfu Xiefei method on intestinal barrier of ALI/ARDS rats

关键词:  通腑泻肺  急性肺损伤  急性呼吸窘迫综合征  肠屏障  大鼠  保护作用

Key words:  Tongfu Xiefei  acute lung injury  acute respiratory distress syndrome  intestinal barrier  rat  protective effect

摘要:

[目的] 探讨通腑泻肺中药对急性肺损伤/急性呼吸窒迫综合征（ALI/ARDS）大鼠肠屏障的保护作用。[方法] 27只大鼠随机分为空白组、模型组、中药组，每组9只。模型组与中药组尾静脉注射内毒素8 mg/kg，空白组大鼠静脉注射生理盐水。中药组在造模完成后给予通腑泻肺中药灌胃，每次0.01mL/g，每日1次，共7日，空白组与模型组予等量生理盐水灌胃。通过病理切片观察各组大鼠肠组织损伤情况，ELISA法检测大鼠血血浆D-乳酸及DAO水平，免疫组化染色方法方法检测肺组织核转录因子-κB（NF-κB） P65及TLR4表达。[结果] 模型组大鼠肠组织镜下可见严重的病理损伤，中药组大鼠肠组织病理损伤减轻。模型组大鼠血浆D-乳酸、DAO明显升高，中药组血浆D-乳酸和DAO降低。模型组大鼠肠组织NF-κB P65和TLR4蛋白表达升高，中药组NF-κB P65和TLR4蛋白表达显著降低。[结论] 通腑泻肺中药对ALI/ARDS病理过程中释放的大量炎性细胞因子有抑制作用，改善ALI/ARDS时肠黏膜的缺血缺氧状态，增加肠黏膜血流灌注，减轻肠黏膜细胞损害，降低肠黏膜通透性，对肠黏膜屏障起到保护作用。

Abstract:

[Objective] To explore the protective effect Tongfu Xiefei herbs on the intestinal barrier of ALI/ARDS rats.[Methods] The 27 rats were randomly divided into blank group, model group and traditional Chinese medicine group, with 9 rats in each group. Endotoxin 8 mg/kg was injected into the tail vein of the model group and the traditional Chinese medicine group. The blank group was injected with normal saline in the rats. After the model was completed, the traditional Chinese medicine group was given the traditional Chinese medicine for diarrhea and diarrhea. Each time was 0.01 mL/g, once a day, for a total of 7 days. The blank group and the model group were given the same amount of normal saline for gastric perfusion. The intestinal tissue injury in each group was observed by pathological sections. The levels of D-lactic and DAO in blood plasma were detected by ELISA. The expression of NF-κB, P65 and TLR4 in lung tissue was detected by immunohistochemical staining.[Results] Severe pathological damage was observed in the intestinal tissue of the rats in the model group, and the pathological damage of the intestinal tissue of the rats in the Chinese medicine group was reduced. The plasma D-lactic acid and DAO in the model group were significantly increased, and the plasma D-lactic acid and DAO were decreased in the Chinese medicine group. The expression of NF-κB P65 and TLR4 protein in the intestinal tissue of the rat model group increased, and the expression of NF-κB P65 and TLR4 protein in the Chinese medicine group decreased significantly.[Conclusion] Tongfu Xiefei herbs on inflammatory cytokine release in the pathological process of ALI/ARDS inhibit the ischemia and hypoxia of ALI/ARDS improve intestinal mucosa, increase intestinal perfusion, reduce cell damage of intestinal mucosa, reduce the intestinal permeability and the protective effect on the intestinal mucosal barrier.