| 摘要: |
| [目的] 探讨复明汤用于肺纤维化治疗的具体作用机制，为其临床应用奠定理论基础。[方法] 通过荧光素酶报告基因检测复明汤对转达化生长因子β1（TGF-β1）信号通路的影响，采用蛋白印记法（Western blot）检测其对TGF-β1诱导的Smad2/3依赖途径和肺Smad信号传导的影响；实时荧光定量聚合酶链反应（qRT-PCR）及Western blot法检测复明汤对细胞外基质（ECM）相关mRNA和蛋白水平及上皮损伤相关mRNA和蛋白的影响，最终构建小鼠肺纤维化模型，明确复明汤对小鼠肺纤维化的治疗效果。[结果] 复明汤可拮抗TGF-β1/Smad3信号传导，其不仅可抑制TGF-β1诱导的肺泡上皮细胞损伤，而且可降低TGF-β1诱导的成纤维细胞分化、细胞外基质生成和成纤维细胞迁移。体内实验表明复明汤可抑制博来霉素诱导的肺损伤，甚至减弱小鼠中已成形的肺纤维化。[结论] 复明汤可通过介导TGF-β1/Smad3通路减轻博来霉素诱导的小鼠肺纤维化，为复明汤治疗肺纤维化的临床应用奠定理论基础。 |
| 关键词: 复明汤 转化生长因子-β1 Smad3 肺纤维化 |
| DOI：10.11656/j.issn.1672-1519.2020.12.20 |
| 分类号:R563.9 |
| 基金项目:西安市科技计划项目（201805103YX11SF37）。 |
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| Fuming decoction inhibits the activation of lung fibroblast and lung epithelial injury to alleviate bleomycin-induced pulmonary fibrosis through regulating TGF-β1/Smad3 pathway |
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YU Honggang1, FU Dahai1, YANG Lisheng1, SONG Chao1, TANG Junfeng2, QIN Xiaojuan3
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1.Department of Pulmonary Diseases, Xi'an Hospital of Traditional Chinese Medicine, Xi'an 710012, China;2.Department of Traditional Chinese Medicine Pharmacology, Shaanxi University of Chinese Medicine, Xianyang 712000, China;3.Department of Respiratory Medicine, Hanzhong People's Hospital, Hanzhong 723000, China
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| Abstract: |
| [Objective] To explore the mechanism of Fuming decoction in the treatment of pulmonary fibrosis,and to lay a theoretical foundation for its clinical application.[Methods] The effect of Fuming decoction on TGF-β1 signaling pathway was detected by luciferase reporter gene,and its effect on TGF-β1-induced Smad2/3-dependent pathway and non-Smad signaling was detected,qRT-PCR and western blot methods were used to detect the effect of fuming decoction on the levels of ECM-related mRNA and protein and epithelial damage-related mRNA and protein. Finally,mouse lung fibrosis model was established to clarify the therapeutic effect of fuming decoction on pulmonary fibrosis.[Results] Fuming decoction can antagonize TGF-β1/Smad3 signaling,which can not only inhibit TGF-β1-induced alveolar epithelial cell damage,but also reduce TGF-β1-induced fibroblast differentiation,extracellular matrix formation and fibroblast migration. In vivo experiments showed that Fuming decoction could inhibit bleomycin-induced lung injury and even attenuate formed pulmonary fibrosis in mice.[Conclusion] Fuming decoction can alleviate bleomycin-induced pulmonary fibrosis in mice by mediating the TGF-β1/Smad3 pathway,which lays a theoretical foundation for the clinical application of fuming decoction in the treatment of pulmonary fibrosis. |
| Key words: fuming decoction TGF-β1 Smad3 pulmonary fibrosis |
