| 摘要: |
| [目的] 观察白藜芦醇（RES）对5-氟尿嘧啶（5-FU）化疗结肠癌敏感性及磷脂酰肌醇激酶（PI3K）/蛋白激酶（Akt）信号通路的影响。[方法] 体外培养SW620细胞，CCK8法检测细胞活力，倒置显微镜分析细胞克隆数，流式细胞术分析细胞凋亡率，蛋白免疫印迹法（Western blot）检测凋亡蛋白Cle-caspase 9、Cle-caspase 7和Cle-PARP及PI3K/Akt信号通路相关蛋白表达水平。[结果] 与对照组比较，RES组、5-FU组和RES+5-FU组SW620细胞活力、克隆数及p-p85、p-110β、p-PDK1和p-Akt表达水平明显降低，而细胞凋亡率及Cle-caspase 9、Cle-caspase 7和Cle-PARP表达水平明显增高（P<0.05）；与RES组和5-FU组比较，RES+5-FU组SW620细胞活力、克隆数及p-p85、p-110β和p-PDK1和p-Akt表达水平明显降低，而细胞凋亡率及Cle-caspase 9、Cle-caspase 7和Cle-PARP表达水平明显增高（P<0.05）。PI3K抑制剂LY294002增强RES和5-FU联合处理对SW620细胞生长的抑制作用，而PI3K基因过表达则降低RES和5-FU联合处理对SW620细胞生长的抑制作用。[结论] RES与5-FU协同抑制PI3K/Akt信号通路诱导结肠癌的化疗效果。 |
| 关键词: 白藜芦醇 结肠癌 5-氟尿嘧啶 PI3K/Akt信号通路 |
| DOI：10.11656/j.issn.1672-1519.2021.10.24 |
| 分类号:R285.5 |
| 基金项目: |
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| Resveratrol synergizes the inhibitory effect of 5-fluorouracil in colon cancer by suppressing PI3K/Akt signaling pathway |
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CUI Yonghe, SHEN Xianmin
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Affiliated Hospital of Hubei University of Arts and Science, Xiangyang Central Hospital, Xiangyang 441021, China
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| Abstract: |
| [Objective] To observe the effects of resveratrol (RES) on the chemotherapeutic sensitivity of 5-fluorouracil(5-FU) in colon cancer and PI3K/Akt signaling pathway.[Methods] SW620 cells were cultured in vitro. CCK8 assay was performed to detect the cell viability. Cell colonies were measured by inverted microscope. SW620 cell apoptotic rate was determined using flow cytometry. The expression levels of apoptosis related proteins including Cle-caspase 9, Cle-caspase 7 and Cle-PARP and proteins in PI3K/Akt signaling pathway were detected by Western Blot. [Results] Compared with control group, the cell viability, colonies and the expression levels of p-p85, p-110β, p-PDK1 and p-Akt in RES group, 5-FU group and RES+5-FU group were significantly decreased, whereas cell apoptosis and the expression levels of Cle-caspase 9, Cle-caspase 7 and Cle-PARP were greatly increased (P<0.05). Compared with RES group and 5-FU group, the cell viability, colonies and the expression levels of p-p85, p-110β, p-PDK1 and p-Akt in RES+-FU group were significantly decreased, whereas cell apoptosis and the expression levels of Cle-caspase 9, Cle-caspase 7 and Cle-PARP were greatly increased (P<0.05). Moreover, PI3K inhibitor (LY294002) promote the inhibitory effect of RES and 5-FU combination on the growth of SW620 cells, whereas PI3K overexpression inhibits it. [Conclusion] RES and 5-FU synergized the chemotherapeutic effect of colon cancer through the suppression of PI3K/Akt signaling pathway. |
| Key words: resveratrol colon cancer 5-fluorouracil PI3K/Akt pathway |
