基于网络药理学探究补肾活血汤治疗肾虚血瘀型膝骨性关节炎的作用机制

Exploration on the mechanism of Bushen Huoxue Decoction on knee osteoarthritis with kidney deficiency and blood stasis based on network pharmacology

关键词:  补肾活血汤  肾虚血瘀  网络药理学  膝骨性关节炎  通路

Key words:  Bushen Huoxue Decoction  kidney deficiency and blood stasis  network pharmacology  osteoarthritis of the knee  pathways

摘要:

[目的] 探究补肾活血汤（BHD）治疗肾虚血瘀型膝骨性关节炎（KOA）的关键靶点以及作用。[方法] 通过Swiss Target Prediction检索BHD中主要活性化合物的相关靶点；GeneCards数据库搜集KOA的疾病靶点，相交得出药物与疾病的共有靶点；将BHD中的主要靶点与活性化合物，使用STRING数据库绘制蛋白互作（ PPI）网络图，对关键靶点进行GO及KEGG通路富集分析。[结果] BHD主要有效活性成分82个，潜在靶点615个；KOA疾病靶点482个，药物基因靶点533个。GO富集分析得到细胞生物学条目119个，续用KEGG通路富集分析方法得到信号通路总数共95个。[结论] 阐述了BHD多成分、多途径、多靶点治疗KOA的分子作用机制，为后续研究及临床提供依据。

Abstract:

[Objective] To explore the key target and effect of Bushen Huoxue Decoction (BHD) in the treatment of knee osteoarthritis (KOA) with kidney deficiency and blood stasis.[Methods] The target of main active compounds in BHD was searched by Swiss Target Prediction. The disease targets of KOA were collected through Genecards database,and the intersection of the two was obtained to obtain a common target. Then,the STRING database was used to plot the protein interaction (PPI),and the GO and KEGG pathway enrichment analysis of key targets were performed.[Results] There were 82 active components and 615 potential targets of BHD. There were 482 targets for KOA diseases and 533 drug diseases. GO enrichment analysis obtained 119 cell biological items;95 related signaling pathways were obtained by KEGG pathway enrichment analysis.[Conclusion] The molecular mechanism of BHD in the treatment of KOA with multiple components,multiple pathways and multiple targets was expounded,which provided ideas for the follow-up research and clinical prescription.