何首乌及炮制品中二苯乙烯苷的稳定性研究

Studies on the stability of stilbene glycoside in crude Polygoni multiflora and its processed products

关键词:  何首乌  炮制  稳定性  顺反异构  降解  聚合

Key words:  Polygoni Multiflori Radix  processing  stability  cis/trans isomerization  degradation  polymerization

摘要:

[目的] 研究何首乌生品及不同炮制品中2，3，5，4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷（TSG）的稳定性。[方法] 采用高效液相色谱（HPLC）及高效液相色谱-质谱联用仪（LC-MS），系统考查了光照、温度和铁离子（Fe³⁺）浓度等因素对TSG单体、何首乌生品及炮制品中TSG的影响。[结果] 各个因素对TSG的稳定性均有不同程度的影响。其中光照、温度等因素对TSG单体、何首乌生品及其炮制品中TSG的影响并不存在明显差别，而Fe³⁺对何首乌生品及其炮制品中反应平衡点存在明显差别，推测可能原因是何首乌中的其他相似结构的二苯乙烯类成分与TSG竞争消耗Fe³⁺所致。[结论] 文章对何首乌指标成分的稳定性影响因素进行了系统研究，为规范其加工、贮藏、炮制和制剂相关研究奠定一定基础，为相似成分的稳定性研究提供了参考。

Abstract:

[Objective] To investigate the stability of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside(TSG) in raw and processed Polygoni multiflora.[Methods] The effects of sunlight,temperature and Fe³⁺ on TSG,raw and processed Polygoni multiflori were systematically investigated by HPLC and LC-MS.[Results] All factors have different effects on the stability of TSG. There was no significant difference among TSG,raw and processed Polygoni multiflori under the sunlight,and high temperature,however,their reaction equilibrium point was significant difference under different Fe³⁺ concentrations. It was speculated that the possible reason was the existence of other stilbenes in Polygoni multiflori.[Conclusion] In this study,the factors which influenced the stability of Polygoni multiflori were systematically studied. The results explored a scientific foundation for the standardization of its processing,storage,and preparation. It also provides a reference for other studies on the stability of similar ingredients.