生脉胶囊对5-氟尿嘧啶致小鼠化疗性肠黏膜炎的保护作用研究

Protective effect of Shengmai Capsule on chemotherapy-induced intestinal mucositis in mice treated with 5-Fluorouracil

关键词:  生脉胶囊  5-氟尿嘧啶  化疗性肠黏膜炎  肠道屏障  杯状细胞

Key words:  Shengmai Capsule  5-Fluorouracil  chemotherapy induced intestinal mucositis  intestinal barrier  goblet cell

摘要:

[目的]探讨生脉胶囊对5-氟尿嘧啶(5-FU)诱导的小鼠结肠黏膜损伤的保护作用。[方法]将20只雄性Balb/c小鼠随机分为正常组、模型组、生脉组。除正常组外,其余2组均采用腹腔注射5-FU(25 mg/kg)诱导建立结肠黏膜损伤小鼠模型。正常组和模型组每日灌胃等量蒸馏水,生脉组每日灌胃等量生脉胶囊悬液(350 mg/kg)。每日监测小鼠体质量和腹泻情况;测量小鼠结肠长度;检测小鼠血清中二胺氧化酶(DAO)活性;苏木精-伊红染色法(HE)观察结肠的组织病理变化;免疫组化法检测小鼠结肠黏膜中ki67表达水平;过典酸雪夫氏染色法(PAS)检测小鼠结肠黏膜中杯状细胞数量。[结果]与模型组比较,生脉组腹泻情况得到明显改善;结肠长度显著增加(P＜0.05);血清中DAO活性显著下降(P＜0.01);结肠黏膜结构明显改善;结肠黏膜中ki67表达显著增加(P＜0.000 1);杯状细胞数量明显增加。[结论]生脉胶囊对5-FU诱导的结肠黏膜损伤具有明显的保护作用。

Abstract:

[Objective] To investigate the protective effect of Shengmai Capsule on colon mucosal damage induced by 5-fluorouracil(5-FU) in mice. [Methods] Twenty male Balb/c mice were randomly divided into normal group,model group and Shengmai group. The mice in model group and Shengmai group received intraperitoneal injection of 5-FU(25 mg/kg) to induce colon mucosal damage. Then,mice in normal group and the model group were given an equal amount of distilled water by gavage,while mice in Shengmai group was given an equal amount of Shengmai capsule suspension(350 mg/kg) by gavage every day. The body weight and diarrhea of mice were monitored every day;the colon length of mice was measured;the activity of diamine oxidase(DAO) in mice serum was detected;histopathological changes of colon were observed by HE(Hematoxylin-eosin staining) staining;the expression level of ki67 in colon tissue was detected by immunohistochemistry;The number of goblet cells in colon tissue was detected by PAS(Periodic Acid Schiff stain) staining. [Results] Compared with the model group,the diarrhea of mice in Shengmai group was improved significantly;the length of colon increased significantly(P＜0.05);The activity of DAO in serum decreased(P＜0.01);the structure of colon mucosa was improved obviously;the expression of ki67 and the number of goblet cells in colon tissue increased(P＜0.000 1). [Conclusion] Shengmai capsule has obviously protective effect on colon mucosal damage induced by 5-FU.