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| 基于网络药理学和体外实验验证探讨八子补肾胶囊延缓年龄相关黄斑变性的作用机制 |
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舒容丽1, 龚博炀1, 李兆栋1, 谭雅文1, 赵雪2, 张佳妮3, 周会芳1, 侯云龙4, 边育红1
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1.天津中医药大学中西医结合学院, 天津 301617;2.新疆医科大学第一临床医学院, 乌鲁木齐 830011;3.黑龙江中医药大学基础医学院, 哈尔滨 150040;4.河北医科大学, 石家庄 050011
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| 摘要: |
| [目的] 基于网络药理学探究八子补肾胶囊（BZBS）延缓年龄相关黄斑变性（AMD）的作用机制并进行体外实验验证。[方法] 通过网络药理学构建BZBS治疗AMD的“中药-疾病-关键靶点”可视化关系图，对关键靶点进行GO分析和KEGG信号通路富集分析。使用BZBS冻干粉干预ARPE-19细胞进行体外实验，通过ROS染色、β-半乳糖苷酶染色（SA-β-gal）、蛋白免疫印迹法（Western blot）、酶联免疫吸附实验（ELISA）等检测其是否能改善D-gal诱导的ARPE-19细胞衰老，并对网络药理学预测的信号通路结果进行验证。[结果] 网络药理学筛选共获得槲皮素、远志酮A、豆甾醇、花生四烯酸等293个BZBS主要活性成分；预测出BZBS与AMD的关键靶点58个。体外实验显示，BZBS能有效提高衰老ARPE-19细胞活力，下调活性氧程度，减少促炎细胞因子分泌，同时上调自噬信号通路关键蛋白P62、LC3的表达（P<0.05）。[结论] BZBS可延缓AMD的衰老进程，其作用机制可能与调控自噬信号通路相关。 |
| 关键词: 八子补肾胶囊 年龄相关性黄斑变性 自噬 细胞衰老 体外实验 视网膜上皮细胞 网络药理学 |
| DOI：10.11656/j.issn.1672-1519.2025.01.19 |
| 分类号:R285.5 |
| 基金项目:健康中国跨领域工程科技未来20年发展战略研究（L2124007）；天津中医药大学中西医结合学院2021年研究生创新基金（ZXYCXLX202107）；天津市教委科研计划项目（2021KJ137）；石家庄市“揭榜挂帅”制科技项目（231790133A）；石家庄市引进高层次科技创新创业人才项目（07202203）；河北省重点研发计划项目（22372502D）。 |
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| Exploring the mechanism of Bazi Bushen Capsule in delaying age-related macular degeneration based on network pharmacology and in vitro experimental validation |
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SHU Rongli1, GONG Boyang1, LI Zhaodong1, TAN Yawen1, ZHAO Xue2, ZHANG Jiani3, ZHOU Huifang1, HOU Yunlong4, BIAN Yuhong1
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1.School of Integrated Traditional Chinese and Western Medicine, Tianjin Universty of Traditional Chinese Medicine, Tianjin 301617, China;2.First Clinical Medical College, Xinjiang Medical University, Urumqi 830011, China;3.Basic Medicine School of Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China;4.Hebei Medical University, Shijiazhuang 050011, China
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| Abstract: |
| [Objective] To investigate the mechanism of Bazi Bushen Capsules（BZBS） in delaying age-related macular degeneration（AMD） based on network pharmacology and validate it in vitro. [Methods] Constructing a visualization relationship diagram of “traditional Chinese medicine-disease-key target” of BZBS in the treatment of AMD through network pharmacology；GO analysis and KEGG signaling pathway enrichment analysis were performed on the key targets. The freeze-dried powder of BZBS was used to intervene in ARPE-19 cells for in vitro experiments，and whether it could improve D-gal-induced ARPE-19 cellular senescence was examined by ROS staining，β-galactosidase staining（SA-β-gal），Western blot，the ELISA. And validate the signaling pathway results predicted by network pharmacology. [Results] The network pharmacology screening yielded a total of 293 main active ingredients of BZBS，including quercetin，farnesolone A，stigmasterol，and arachidonic acid；the 58 key targets of BZBS and AMD were predicted. in vitro experiments showed that BZBS could effectively increase the viability of senescent ARPE-19 cells，down-regulate the degree of reactive oxygen species，reduce the senescence-related secretion phenotype，and up regulate the expression of P62，and LC3，key proteins of autophagy signaling pathway（P<0.05）. [Conclusion] BZBS can delay the aging process of AMD，and its mechanism may be related to the regulation of autophagy signaling pathway. |
| Key words: Bazi Bushen Capsule age-related macular degeneration autophagy cellular aging in vitro experiment retinal epithelial cell network pharmacology |
