黄芪桂枝五物汤改善环磷酰胺致生精障碍小鼠的肠道菌群紊乱

Study on the effects of Huangqi Guizhi Wuwu Decoction on the gut microbiota dysbiosis in mice with oligoasthenospermia induced by cyclophosphamide

关键词:  黄芪桂枝五物汤  生精障碍  生精功能  肠道菌群

Key words:  Huangqi Guizhi Wuwu Decoction  oligoasthenospermia  spermatogenic function  gut microbiota

摘要:

[目的] 基于肠道菌群探究黄芪桂枝五物汤治疗少精子症的机制，并通过生精障碍动物实验进行初步验证。[方法] 采用环磷酰胺造模小鼠生精障碍模型，研究黄芪桂枝五物汤对生精功能及肠道菌群的调节作用。实验随机选取10只昆明小鼠为正常组，连续7 d腹腔注射生理盐水；其余小鼠连续7 d腹腔注射环磷酰胺[60 mg/（kg·d）]制备生精障碍模型，而后随机分为模型组、黄芪桂枝五物汤低、中、高剂量组（8、16、32 g/kg），每组10只。药物灌胃干预35 d后，记录小鼠睾丸指数、附睾指数；采用计算机辅助精子分析系统检测附睾中精子质量；苏木素-伊红染色观察睾丸组织形态，并采用 16S rDNA 高通量测序技术分析小鼠的肠道菌群组成。[结果]黄芪桂枝五物汤可提高生精障碍模型小鼠的睾丸系数和附睾系数，增加精液浓度和精子活力，减轻睾丸组织病理损伤程度，改善肠道菌群结构，使得Parabacteroides丰度增加、Roseburia丰度减少。[结论] 表明黄芪桂枝五物汤改善环磷酰胺导致的小鼠生精障碍和肠道菌群紊乱。

Abstract:

[Objective] To study the mechanism of Huangqi Guizi Wuwu Decoction（HGW） on alleviating the pathological changes caused by oligoasthenospermia，based on the modification of gut microbiota. [Methods] Normal Kunming mice were selected as control group，which received an 7-days injection of saline；the other mice were injected by 60 mg/（kg·d） I.p Cyclophosphamide continuously for seven days，which were divided into oligoasthenospermia model group and HGW low，middle and high dose treatment group（8，16，32 g/kg）. Each group contained ten mice. H&E staining was used to observe the pathological changes in testis. The gut microbiota was detected by 16S rDNA sequencing. The body weight and testis quality would be recorded after 35 days intragastric administration. The sperm quality would be tested by computer-aided sperm analysis（CASA） software. [Results] AThe results showed that HGW greatly enhanced testis and epididymis index，significantly increased sperm quality and viability，reduced pathological lesions of testicular tissue in mice with oligoasthenospermia. Furthermore，they could also reverse the gut microbiota disorder，increase the relative abundance of Parabacteroides，decrease the relative abundance of Roseburia. [Conclusion] These results suggested that HGW could mprove the spermatogenic function and gut microbiota dysbiosis in mice with oligoasthenospermia induced by cyclophosphamide.