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| 基于UPLC-Q-TOF-MS/MS技术的冠心静化学成分分析 |
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刘阳1,2, 林文涵1, 李雯雯1, 任海3, 赵娇3, 欧阳慧子1,2, 何俊1
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1.天津中医药大学, 组分中药国家重点实验室, 天津 301617;2.天津中医药大学第一附属医院, 天津 300193;3.保定中药制药股份有限公司, 安国 071205
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| 摘要: |
| [目的] 对冠心静的化学成分进行快速分析与鉴定。[方法] 应用超高效液相色谱-四极杆-飞行时间串联质谱技术（UPLC-Q-TOF-MS/MS），结合特征碎片和中性损失系统的表征鉴定冠心静中的化学成分。[结果] 根据精确相对分子质量和二级质谱碎片离子信息，并结合对照品与文献数据，共从冠心静中鉴定出96个主要化学成分，包括29个萜类、29个有机酸类、11个苯酞类、13个黄酮类、14个其他类化合物。[结论] 该方法能够快速、准确地分析冠心静中的化学成分，为冠心静的质量控制和药效物质研究提供了依据。 |
| 关键词: 冠心静 超高效液相色谱-四极杆-飞行时间串联质谱法 化学成分 定性分析 |
| DOI：10.11656/j.issn.1672-1519.2025.03.12 |
| 分类号:R284.1 |
| 基金项目:国家自然科学基金项目（82274091）。 |
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| Identification of chemical constituents in Guanxinjing based on UPLC-Q-TOF-MS/MS technology |
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LIU Yang1,2, LIN Wenhan1, LI Wenwen1, REN Hai3, ZHAO Jiao3, OUYANG Huizi1,2, HE Jun1
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1.State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;3.Baoding Traditional Chinese Medicine Pharmaceutical Co., Ltd., Anguo 071205, China
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| Abstract: |
| [Objective] To rapidly analyze and identify the chemical constituents of Guanxinjing. [Methods] Based on UPLC-Q-TOF-MS/MS technology，the chemical constituents in Guanxinjing were systematically characterized and identified by their characteristic fragments and neutral losses. [Results] A total of 96 major constituents including 29 terpenoids，29 organic acids，11 phthalides， 13 flavonoids，and 14 other constituents were identified by compared the accurate mass measurements and secondary mass spectrometry fragment ions，and combined with the literature report and the reference. [Conclusion] This method can quickly and accurately analyze the chemical constituents of Guanxinjing，which provides a theoretical basis for the quality control and pharmacodynamic substances research of Guanxinjing. |
| Key words: Guanxinjing ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry chemical constituents qualitative analysis |
