醒脑开窍针法对卒中后抑郁模型大鼠行为学改变及单胺类神经递质释放的影响

Effects of Xingnao Kaiqiao acupuncture on behavioral changes and release of monoamine neurotransmitters in post-stroke depression model rats

关键词:  抑郁  卒中后  行为学  单胺类神经递质  醒脑开窍  大鼠

Key words:  depression  after stroke  behavioral science  monoamine neurotransmitters  Xingnao Kaiqiao  rat

摘要:

[目的] 探讨醒脑开窍针刺对卒中后抑郁（PSD）大鼠行为学改变及单胺类神经递质释放的影响。[方法] 将实验的SD大鼠56只，随机分为正常组（14只）、模型组（14只）、药物组（14只）、针刺组（14只）。除正常组外，其余组42只大鼠均采用Longa线栓法和慢性不可预知性温和应激（CUMS）法复合制备PSD模型，并剔除建模过程中建模失败和死亡的大鼠。药物组给予氟西汀灌胃治疗，针刺组利用醒脑开窍针刺法进行针刺处理，正常组和模型组给与生理盐水1 mL灌胃。对比造模前（0 d）、CUMS前（7 d）、治疗前（14 d）、治疗后（35 d）大鼠的神经功能缺损评分、体质量、旷场实验垂直得分和水平得分、糖水消耗量和血清中单胺类神经递质释放水平。[结果] 重复测量组内不同时间点比较，神经功能缺损评分（P<0.001），时间与不同组别比较差异具有统计学意义（P<0.01）。组内不同时间点比较，体质量比较差异具有统计学意义（P<0.001），时间与不同组别之间存在交互作用（P<0.01）。组内不同时间点比较，旷场实验垂直得分和水平得分比较差异有统计学意义（P<0.001），时间与不同组别之间存在交互作用（P<0.01）。组内不同时间点比较，糖水消耗量比较差异有统计学意义（P<0.001），时间与组别之间存在交互作用（F=3.531，P<0.01）。治疗后（35 d）与正常组相比，模型组中5-羟色胺（5-HT）、多巴胺（DA）和去甲肾上腺素（NE）水平显著降低（P<0.05），与模型组相比，药物组和针刺组中5-HT、DA和NE水平明显升高（P<0.05）。[结论] 醒脑开窍针刺在改善行为学和神经递质方面与氟西汀效果相当，对神经功能损伤具有一定的恢复作用。

Abstract:

[Objective] To investigate the effects of Xingnao Kaiqiao acupuncture on behavioral changes and release of monoamine neurotransmitters in post-stroke depressed（PSD）rats. [Methods] A total of 56 SD rats were randomly divided into a normal group（14 rats）， a model group（14 rats），a drug group（14 rats），and an acupuncture group（14 rats）. Except for the normal group，the remaining 42 rats were used to prepare the PSD model using the Longa line occlusion method and the chronic unpredictable mild stress（CUMS）method， and rats that failed to model and died during the modeling process were excluded. The drug group was given fluoxetine intragastric treatment. The acupuncture group was treated by Xingnao Kaiqiao acupuncture，and the normal group and model group were given 1 mL normal saline intragastric treatment. Neurologic deficit score，body weight，vertical score and horizontal score in open field test，sugar water consumption and serum monoamine neurotransmitter release level were compared before modeling（0 d），before CUMS（7 d）， before treatment（14 d）and after treatment（35 d）. [Results] According to the ANOVA results of repeated measurement data，there were statistically significant differences in neurological impairment scores at different time points in the group（P<0.001），and there was an interaction between time and group（P<0.01）. The results indicated that the variation trend of neural function deficit score in different groups was different with time. There was a statistical difference in body weight at different time points in the group（P<0.001），and there was an interaction between time and group（P<0.01），suggesting that the change trend of body weight in different groups was different with time. Compared at different，differences between the vertical scores and horizontal scores in open field experiment（P<0.001），and there was an interaction between time and group（P<0.01）. The results indicated that the vertical and horizontal scores of rats in different groups were different over time. Compared at different time points in the group，there were statistical differences in sugar water consumption（P<0.001）. There was an interaction between time and group（P<0.01），suggesting that the change trend of sugar water consumption in different groups was different with time. After treatment（35 d），the levels of 5-Hydroxytryptamine（5-HT），Dopamine（DA）and Norepinephrine（NE）in the model were significantly decreased（P<0.05），and the levels of 5-HT，DA and NE in the drug group and acupuncture group were significantly increased compared with the model group（P<0.05）. [Conclusion] Acupuncture for awakening the brain and opening the mind is comparable to the effect of fluoxetine in improving behavioral and neurotransmitter aspects. The method of acupuncture has a certain recovery effect on nerve function injury.