中药清解灵配合胆管减压引流对急性重症胆管炎大鼠脏器中磷脂酶A2和氧自由基含量的影响*

The effect of Qingjieling combined with choledochostomyon the content of phospholipase A2 and oxygen free radical in organic tissue of rats with acute cholangitis of severe type

关键词:  急性重症胆管炎  氧自由基  磷脂酶A₂  清解灵

Key words:  Acute cholangitis of severe type (ACST)  Oxygen Free Radical (OFR)  Phospholipase A₂(PLA₂)  Qingjieling

摘要:

[目的]观察中药清解灵配合胆管减压引流对急性重症胆管炎大鼠脏器组织中磷脂酶A₂(PLA₂)和氧自由基(OFR)含量的影响。[方法]制造胆道急性感染模型和胆管减压内引流模型。实验大鼠随机分为正常、ACST、减压和中药4组。采用电子自旋共振仪检测组织中的OFR含量。采用酸滴定法检测组织中PLA₂活性。[结果]急性重症胆管炎大鼠回肠黏膜、肝、肺组织中的OFR含量,与正常组比较显著升高(P值分别为:<0.05,<0.01,<0.001),PLA₂活性与正常组比较明显增强(P值分别为:<0.05,<0.01,<0.001)。与ACST组比较,中药组OFR含量和PLA₂活性降低为著(P值均<0.05)。[结论]中药清解灵配合胆管减压引流治疗,可有效地降低脏器中的炎症介质OFR含量和PLA₂活性,减轻由于过度炎症反应所造成的损伤,从而起到保护组织器官的作用。

Abstract:

[Objective] To investigate the effect of Qingjieling combined with choledochostomy on the content of phospholipase A₂ (PLA₂) and oxygen free radical (OFR) in organic tissues of rats with acute cholangitis of severe type (ACST).[Methods] The rat models with ACST and choledochostomy are made. Subject rats are randomly divided into four groups: the normal group, ACST group, choledochostomy group, and medicinal-herb group. The content of OFR and the activity of PLA₂ of tissues are determined by using the electron rotating resonation technique and acidometrictitration respectively.[Results] Compared with the normal group, the content of OFR in ileomucosa, hepatic and pulmonary tissues in ACST and choledochostomy group both increased markedly (P<0.05, <0.01, <0.001, separately);the activities of PLA₂ were greatly raised (P<0.05,<0.01,<0.001, separately). Compared with the ACST group, either the content of OFR or the activity of PLA₂ in the medicinal-herb group decreased remarkably (P<0.05,without exception).[Conclusion]: The therapy of Qingjieling combined with choledochostomy can significantly reduce inflammatory media, including OFR and PLA₂ in many tissues of rats with acute cholangitis of severe type so as to protect organs from the damage of over-inflammation.