首乌丹参滴丸对实验性动脉粥样硬化家兔ET/NO及NOS的影响*

Influence of Shouwu Danshen Dropping Pills on ET/NO and NOS in Rabbits with Experimental Atherosclerosis

关键词:  动脉硬化  内皮素  一氧化碳  一氧化氮合酶

Key words:  atherosclerosis  endothelin  nitric oxide  nitric oxide synthase

摘要:

[目的]探讨首乌丹参滴丸(SWDS)对实验性动脉粥样硬化(AS)家兔血内皮素(ET)、血浆一氧化氮(NO)水平和一氧化氮合酶(NOS)的影响,并探讨其对动脉粥样硬化的治疗作用。[方法]取50只健康雄性日本大耳白家兔,随机取10只作为正常对照组,喂普通饲料,其余40只给予高脂饮食,喂养4周后,再随机分为4组,每组10只:模型组继续喂高脂饲料:小剂量治疗组喂以高脂饲料和首乌丹参滴丸生药,每只1.25g(/kg·d),大剂量治疗组每只5g(/kg·d):阳性药物对照组,喂以高脂饲料和辛伐他汀,每只2.35mg(/kg·d)。4周后,测定血脂、血清NO和血浆ET水平及血管壁组织NOS的含量。[结果]小剂量、大剂量治疗组及阳性药物对照组的血浆内皮素-1(ET-1)水平明显较模型组低(P<0.05),血清NO水平及血管壁NOS的表达呈相反变化(P<0.05)。[结论]首乌丹参滴丸可降低血脂和血浆ET-1水平,提高血清NO及动脉壁组织NOS的表达,具有抗动脉粥样硬化作用。

Abstract:

[Objective]To explore the influence of ShouwuDanshen (fleeceflower stem and root of red-rooted salvia) dropping pills (SWDS) on the level of endothelin (ET)、nitric oxide (NO) and nitric oxide synthase (NOS) in rabbits with atherosclerosis and to investigate the therapeutic effect of SWDS on atherosclerosis.[Methods]Fifty healthy male Japanese white rabbits were used in the experiment.Ten rabbits were randomly selected as normal control group and were fed with normal forage.Forty rabbits were fed with hypercholesterol forage for four weeks and then were randomly divided into four groups:model group,small dosage group,large dosage group and control group.The rabbits of model group were only fed with hypercholesterol diet.The rabbits of small dosage group were treated with SWDS 1.25 g/(kg·d) and hypercholesterol diet.The rabbits of large dosage group were treated with SWDS 5 g/(kg·d) and hypercholesterol diet.The rabbits of positive control group were treated with simvastatin 2.35 mg/(kg·d) and hypercholesterol diet.After four weeks the levels of blood lipid,serum NO,plasma ET and NOS content in blood vessel tissue were measured.[Results] The level of plasma ET-1 in small dosage group,large dosage group and control group was significantly less than that in model group (P<0.01).The expression of serum NO and NOS in blood vessel tissue showed opposite change (P<0.05).[Conclusion] The SWDS dropping pills possesses an anti-atherosclerosis effect by decreasing the level of plasma lipids and ET-1 and increasing the expression of serum NO and NOS in blood vessel tissue.