复方苦参注射液对人体子宫颈癌HeLa细胞增殖及雌激素受体ER-α36表达的影响*

Effects of compound Kushen injection on proliferation of HeLa cells and expression of estrogen receptor-α36 in human cervical cancer

关键词:  复方苦参注射液(岩舒)  子宫颈癌  抗肿瘤  雌激素受体  实验研究

Key words:  compound Kushen injection(Yanshu)  human cervical cancer  anticancer  estrogen receptor  experimental study

摘要:

[目的]探讨复方苦参注射液(岩舒)对人子宫颈癌HeLa细胞新型雌激素受体(ER)亚型ER-α36的影响。[方法]采用四甲基偶氮唑蓝(MTT)法观察不同浓度复方苦参注射液及复方苦参注射液联合顺铂(DDP)对人子宫颈癌HeLa细胞增殖的影响;采用逆转录聚合酶链反应法(RT-PCR)检测复方苦参注射液对人子宫颈癌HeLa细胞中新型雌激素受体ER-α36表达水平的影响。[结果]复方苦参注射液可以抑制HeLa细胞增殖,并呈剂量和时间依赖性;复方苦参注射液与DDP联合使用时,表现出其对DDP的增效作用;复方苦参注射液,特别是其与顺铂联合用药作用于HeLa细胞48 h后,细胞中ER-α36在mRNA表达水平明显降低,与空白对照组相比差异有统计学意义(P<0.05)。[结论]复方苦参注射液可抑制人子宫颈癌HeLa细胞增殖;并可增强DDP抗肿瘤作用;复方苦参注射液及其联合用药组可明显抑制ER-α36表达,而联合用药组作用更佳。

Abstract:

[Objective] To explore the effects of compound Kushen injection (Yanshu) on the estrogen receptor-α36 in HeLa cells of human cervical cancer.[Methods] We used MTT method to detect the inhibitory effect on HeLa cell proliferation after treating by compound Kushen injection,DDP and its combinations.Using RT-PCR method the expression of ER-α36 after treating by compound Kushen injection was detected.[Results] Compound Kushen injection could inhibit the proliferation of HeLa cell and the effect was time and dose dependent.Compound Kushen injection could enhance the effect of DDP.The expression of HeLa cell ER-α36 on mRNA level after treating for 48 h by compound Kushen injection,the combination of compound Kushen injection and DDP,was reduced significantly (P<0.05).[Conclusion] Compound Kushen injection can inhibit the proliferation of HeLa cell and enhance the anticancer effect of DDP.Compound Kushen injection can reduce the expression of HeLa cell ER-α36 on mRNA level.The combined treating with these has more effective anti-tumor effect than Kushen injection.