脑梗死急性期局部血流量变化及电针干预效应研究*

Effect of electroacupuncture on rCBF of rats with acute cerebral infarction

关键词:  脑梗死  电针  局部脑血流量  激光多普勒

Key words:  cerebral infarction  electroacupuncture  regional cerebral blood flow  laser doppler

摘要:

[目的]观察脑梗死发生后双侧脑皮质血流量变化规律以及电针的干预效应。[方法]采用Longa的腔内线栓法复制大鼠脑梗死模型,以DRT-4激光多普勒血流仪动态监测双侧脑皮质血流量,连续记录6h,比较脑梗死后30min和1、2、3、4、5、6h各时相脑皮质血流量的变化规律及电针的干预效应。[结果]大脑中动脉梗死(MCAO)即刻,梗死半球局部脑血流量显著下降,模型对照组局部血流量下降至(25.18±1.87)%,在MCAO后30min~6h,血流量比值维持在这一水平,有上升趋势但本组各时相之间比较并无统计学差异;电针干预组在MCAO即刻局部脑血流量急剧下降至(23.46±1.24)%,电针干预后,平均上升至(46.96±1.61)%,平均升高幅度为20%。在MCAO后各时相,电针干预组与模型对照组血流量比值相比均有统计学差异(P<0.001),电针干预组局部血流量显著升高,但仍然低于梗死前水平(P<0.01)。MCAO即刻,梗死灶对侧半球局部脑血流量出现一定幅度的下降,模型对照组局部脑血流量下降至(86.55±1.46)%,在MCAO后30min~6h内,随时间延续,血流量维持梗死即刻水平,有小幅波动,但本组各时相之间比较无统计学差异;电针干预组在MCAO即刻局部脑血流量下降至(86.70±2.21)%,电针干预后,血流量上升至(97.46±3.28)%,维持这一水平。与模型对照组比较,电针干预组局部脑血流量比值在MCAO后30min和1h存在统计学差异(P<0.05),在MCAO后2、3、4、5、6h局部血流量比值比较无统计学差异,但电针干预组局部脑血流量比值平均高于相应模型对照组。[结论]电针干预可显著提高MCAO模型大鼠局部脑血流量,并且电针干预可同步提高梗死灶对侧半球局部脑血流量,由此可增加梗死区周围脑血流灌注。

Abstract:

[Objective] To investigate the effect of electroacupuncture on rCBF of rats with acute cerebral infarction.[Methods] The acute cerebral infarction was induced by middle cerebral artery occlusion and using laser doppler blood flow assessment the change of regional blood flow after cerebral infarction (every half to one hour for 6 hours after MCAO) was detected in both hemisphere.[Results] Immediately after MCAO, the ipsilateral rCBF was significantly decreased to (25.18±1.87)% and (23.46±1.24)% after intervention of electric acupuncture. The rCBF was also determinately decreased to (86.55±1.46)% and (86.70±2.21)% after intervention in electroacupuncture group immediately at MCAO. After electroacupuncture stimulus, there is a significant increase of the ipsilateral rCBF compared with control group[(46.96±1.61)% and (26.19±1.97)%, P<0.001)], but it was still lower than normal level, and the contralateral rCBF was also increased approximately to normal level in the treatment group. There was significant difference at 0.5 and 1 hour after MCAO compared with that in control group (P<0.05).[Conclusion] Electroacupuncture stimulus can significant enhance rCBF in both hemisphere after MCAO, thereby increasing cerebral blood flow perfusion in the peri-ischemic zone and may also increase cerebral blood flow perfusion to the infarct core.