| 摘要: |
| [目的]优化清开灵口服液提取醇沉工艺条件。[方法]高效液相色谱(HPLC)法监测指标成分栀子苷,采用L9(34)正交实验法,考察水提取和醇沉工艺。[结果]清开灵口服液优化工艺条件为:取处方量栀子、板蓝根和金银花,用9倍量水浸泡药材1h后提取2次,每次2h,合并2次提取液浓缩至生药量与浓缩液体积比1:1,加入95%乙醇调节乙醇浓度为70%,调pH值为6进行醇沉;栀子苷的转移率为64.8%。[结论]该优选工艺重现性好,栀子苷转移率高,固形物收率适宜,达到了生产工艺优化的目的。 |
| 关键词: 清开灵口服液 栀子苷 HPLC 正交实验 提取纯化 |
| DOI：10.11656/j.issn.1673-9043.2012.02.13 |
| 分类号: |
| 基金项目:* 科技部国际合作项目(2008DFB30070);天津市高教科技发展基金项目(0090209) |
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| Optimization in preparation process of Qingkailing oral liquid |
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WANG Lei1, GAO Hui2, CHAN Ai-fu2, LIU Er-wei1, WANG Jia-long1
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1.TCM Chemistry and Analysis Key Laboratory of Tianjin, Tianjin 300193, China;2.Pharmaceutical Co. Ltd of Tangshan Rongda, Tangshan 063020, China
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| Abstract: |
| [Objective] To optimize the condition for the extraction and alcohol-purification process of Qingkailing oral liquid.[Methods] Monitoring geniposide by HPLC detection means,the orthogonal design L9 (34) was used for the inspection of water-extracting process and alcohol-precipitating process.[Results] The condition for the extraction and purification process of Qingkailing oral liquid was as follows: soaking the medicinal materials with 9 folds of water for 1 hour, decocting 2 times for 2 hours. Both alcohol precipitated solutions were concentrated to the concentration of 1:1(material:solution volume), then added 95% alcohol,precipitated by 70% alcohol solution, moderated pH to 6; the extraction rate of geniposide was 64.8%.[Conclusion] The optimum process is repeatable, the extraction rate of geniposide is high, recovery rate of solids are suitable. This research result has attained the goal of optimizing production process. |
| Key words: Qingkailing oral liquid geniposide HPLC orthogonal test extraction and purification |
