育泡饮对大鼠卵巢颗粒细胞间隙连接蛋白43表达及分泌功能的影响*

Effects of Yupao Yin on expression of CX43 in ovarian granulosa cells of rats and secretion function

关键词:  育泡饮  卵巢颗粒细胞  间隙连接蛋白43  雌二醇  实验研究

Key words:  Yupao Yin  ovarian granulosa cells  connexin43  E₂  experimental study

摘要:

[目的]观察育泡饮对体外培养大鼠卵巢颗粒细胞(GC)间隙连接蛋白43(CX43)表达及雌二醇(E₂)分泌的影响,探索中药促进卵泡发育的可能作用机制。[方法]制备大鼠卵巢颗粒细胞进行原代培养,细胞培养48h后,加入促卵泡激素(FSH)50ng/mL和不同剂量的育泡饮,继续培养48h,放免法测定颗粒细胞培养液中E₂的含量,Westernblot法检测CX43蛋白的表达。[结果]1)育泡饮能够促进卵巢颗粒细胞分泌E₂,随着药物浓度的增大,E₂分泌逐渐增加。2)育泡饮可明显促进颗粒细胞CX43蛋白的表达,其中高剂量组的促进作用与FSH组作用相似。[结论]育泡饮能明显促进卵巢颗粒细胞CX43蛋白的表达及E₂分泌,说明育泡饮可通过上调CX43蛋白的表达促进颗粒细胞的增殖,增强颗粒细胞的抗凋亡能力,进而调节卵泡的发育。

Abstract:

[Objective] To assess the effects of Yupao Yin on expression of CX43 in ovarian granulosa cells(GC) of rats and secretion quantity of E₂.[Methods] Granulosa cells are isolated from rats ovaries and cultured for 48 hours,followed by culture in medium system containing FSH(50 ng/mL)and different dosage of Yupao Yin for 48 hours.The expression of CX43 is measured by western blot and the secretion quantity of E₂ is performed by radioimmunoassay.[Result] Yupao Yin can promote secretion of E₂ and the secretion quantity of E₂ gradually increases with the increase of the dosage.Yu pao Yin can significantly stimulate the expression of CX43.Amomg them the effect of high dosage group is similar with FSH group.[Conclusion] Yupao Yin obviously promote the expression of CX43 and secretion of E₂ of ovarian GC of rats.It is showed that Yupao Yin can promote proliferation of GC and enhance the ability of GC anti-apoptosis through up-regulation the expression CX43.And then to promote the development of follicular.