| 摘要: |
| [目的]研究金黄膏在大鼠体内的经皮渗透性。[方法]应用经皮微渗析在体取样技术,以Wistar大鼠为模型动物进行实验。微渗析探针的在体回收率采用反向渗析法测定。[结果]反向渗析法测定的在体回收率为(32.81±1.33)%。渗析液中的药物稳态流量(Js)为2.73ng/h,滞后时间(Tlag)为1.50h。[结论]金黄膏中的有效成分在皮肤内有一定的滞留量,该制剂适合经皮给药。 |
| 关键词: 经皮微渗析 盐酸小檗碱 经皮吸收 高效液相色谱法 |
| DOI：10.11656/j.issn.1673-9043.2012.03.07 |
| 分类号: |
| 基金项目:*国家自然基金项目(81072818) |
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| Preliminary study on permeability of Jinhuanggao by transcutaneous microdialysis in rats in vitro |
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ZHANG Qian1, YIN Zhong-peng1, ZHAO Hai-nan1, PANG Xiao-chen1, MA Li1, GUO Lin2, LIU Zhi-dong1, WANG Hong2
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1.Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin 300193, China;2.Second Affiliated Hospital of Tianjin University of TCM, Tianjin 300150, China
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| Abstract: |
| [Objective] To study the transcutaneous absorption of Jinhuanggao in rats.[Methods] An in vivo sampling technique and transcutaneous microdialysis were used to determine the drug concentration in dermis in rats.The recovery of the microdialysis probe was adopted to assess in vivo according to the retrodialysis method.[Results] The recovery in vivo was(32.81±1.33)% by retrodialysis method.The steady state flux of berberine hydrochloride in dialysate was 2.73 ng/h,and the lag time was 1.5 h.[Conclusion] The active ingredient in Jinhuanggao has a good retention amount under the skin.So this preparation is suitable for transdermal drug delivery. |
| Key words: cutaneous microdialysis berberine hydrochloride transdermal delivery HPLC |
