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| 虫草素抑制人胃癌细胞MKN-28运动侵袭能力研究 |
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孙光斌1, 兰岚2, 唐红梅3, 李宁4, 王悦4
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1.天津市第五中心医院消化内科, 天津300450;2.天津医科大学肿瘤医院中西医结合科, 国家肿瘤临床医学研究中心, 天津300060;3.河北联合大学生命科学学院, 唐山063000;4.南开大学医学院免疫学室, 天津300071
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| 摘要: |
| [目的] 探讨虫草素对人胃癌细胞MKN-28体外迁移、侵袭能力的影响及其可能作用的分子机制。[方法] 悦悦运8法检测虫草素对MKN-28 细胞的增殖抑制作用，体外迁移、侵袭实验观察虫草素对人胃癌细胞MKN-28 迁移、侵袭能力的影响，反转录聚合酶链反应（RT-PCR）法检测虫草素作用24 h后MKN-28 细胞Ezrin 蛋白，基质金属蛋白9（MMP9）信使核糖核酸（mRNA）表达变化。[结果] 200～800 μg/mL 虫草素可明显抑制MKN-28 胃癌细胞增殖，体外迁移实验显示虫草素组细胞划痕闭合约18% ，对照组细胞划痕闭合约25% ，而表皮生长因子（EGF）组闭合约48% 。虫草素组显著低于另两组（P<0.05）。体外侵袭实验结果表明虫草素组穿过人工基底膜的细胞数明显减少（P<0.05）；虫草素组Ezrin 和MMP9 的表达明显下调（P<0.05）。[结论] 虫草素可能通过下调Ezrin 及MMP9 表达抑制人胃癌细胞MKN-28 细胞的迁移与侵袭能力，从而发挥其抑制肿瘤生长的作用。 |
| 关键词: 虫草素 胃癌 MKN-28细胞 Ezrin蛋白 基质金属蛋白9 |
| DOI：10.11656/j.issn.1673-9043.2014.03.07 |
| 分类号: |
| 基金项目:国家自然科学基金项目（81171975）；天津市滨海新区卫生科研基金项目（2011BHKY010）；河北省自然科学基金项目（H2013209194）。 |
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| Effects of Cordyceps sinensis on the migration and invasion capability of human gastric adenocarcinoma cell line MKN-28 |
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SUN Guang-bin1, LAN Lan2, TANG Hong-mei3, LI Ning4, WANG Yue4
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1.Department of Gastroenterology, the Fifth Central Hospital of Tianjin, Tianjin 300450, China;2.Department of Integrated Traditional and Western Medicine, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, China;3.College of Life Science, Hebei United University, Tangshan 063000, China;4.Department of Immunology, School of Medicine, Nankai University, Tianjin 300071, China
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| Abstract: |
| [Objective] To explore the effects of Cordyceps sinensis on the migration and invasion capability and on the expression of Ezrin and MMP9 in human gastric adenocarcinoma cell line MKN-28. [Method] The inhibitory effects of Cordyceps sinensis on the proliferation of MKN-28 cell line were evaluated by CCK-8 assay. The effects of Cordyceps sinensis on the migration and invasion ability of the cells were observed through scratch assay and Boyden chamber, respectively. RT-PCR system detecting the mRNA gene expression changes of Ezrin and MMP9 in MKN-28 cell line. [Result] Cordyceps sinensis from 200 μg/mL to 800 μg/mL can obviously inhibited the proliferation of the human gastric adenocarcinoma cell line MKN-28. Scratch and matrigel invasion experiments showed that cordyceps sinensis significantly inhibited cell migration and invasion. Cordyceps sinensis inhibited the gene expression of Ezrin and MMP9 in MKN-28 cell line. [Conclusion] Cordyceps sinensis may inhibited cell proliferation, migration and invasion through down regulating the expression of Ezrin and MMP9, then suppressing the tumor growth. |
| Key words: Cordyceps sinensis gastric adenocarcinoma MKN-28 cell line Ezrin MMP9 |
