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| 苦豆子多糖羧甲基化修饰及其抗氧化活性的研究 |
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申林卉1,2, 刘丽侠1,2, 陈冠1,2, 赵永安3,2, 陶遵威2
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1.天津中医药大学, 天津300193;2.天津市医药科学研究所, 天津300020;3.天津医科大学, 天津300070
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| 摘要: |
| [目的] 以苦豆子多糖为原料制备羧甲基苦豆子多糖。[方法] 以羧甲基取代度为指标，应用L9（34）正交实验设计对苦豆子羧甲基化工艺进行优化。[结果] 苦豆子多糖50 mg 在氯乙酸钠0.5 g，反应温度55℃，反应时间5 h，氢氧化钠浓度25% 的优化条件下，羧甲基苦豆子多糖取代度DS 可达1.50 以上。[结论] 此制备工艺有效。羧甲基化修饰后的苦豆子多糖清除羟基自由基能力增强。 |
| 关键词: 苦豆子多糖 羧甲基化修饰 取代度 正交实验 抗氧化性 |
| DOI：10.11656/j.issn.1673-9043.2014.03.08 |
| 分类号: |
| 基金项目:天津市自然科学基金项目（10JCZDJC21000）。 |
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| Carboxymethyl modification and antioxidant activity of sophora alopecuroides polysaccharide |
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SHEN Lin-hui1,2, LIU Li-xia1,2, CHEN Guan1,2, ZHAO Yong-an3,2, TAO Zun-wei2
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1.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Tianjin Institute of Medical Pharmaceutical Sciences, Tianjin 300020, China;3.Tianjin Medical University, Tianjin 300070, China
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| Abstract: |
| [Objective] Carboxymethylated sophora alopecuroides polysaccharide was prepared by sophora alopecuroides polysaccharide as the raw material. [Methods] The method was optimized by L9(34) orthogonal test with the substitution degree of carboxymethylation as the index. [Results] The results showed that the optimized condition 50 mg of Sophora alopecuroides polysaccharide was reacted with 0.5 g of Sodium chloroacetate,with reaction temperature of 55℃, reaction time of 5 h, 25% of sodiumhydroxide concentration. The maximum substitution degree (DS) was 1.50 at least. [Conclusion] The preparation process is effective. Meanwhile, carboxymethylated sophora alopecuroides polysaccharide has more accelerated scavenging capacities against hydroxyl, which is much better than that before carboxymethylation. |
| Key words: Sophora alopecuroides polysaccharide carboxymethylation substitution degree orthogonal test antioxidation activity |
