清热化痰法对AECOPD痰热证大鼠模型病理肺组织的影响

The influence of clearing heat and dispersing phlegm method on the pathological lung tissue of AECOPD rat model (syndrome of phlegm and heat)

关键词:  清热化痰法  AECOPD痰热证大鼠模型  病理肺组织

Key words:  clearing heat and dispersing phlegm way  rat model of exacerbation chronic obstructive pulmonary disease (syndrome of phlegm and heat)  pathological lung tissue

摘要:

[目的] 基于前期研究清热化痰方可以提高青霉素在慢性阻塞性肺疾病急性加重期(AECOPD)痰热证大鼠模型肺组织中药物浓度基础上,研究清热化痰方是否通过改善AECOPD受损肺组织的形态结构来利于抗生素在肺组织内的转运。[方法] 50只Wistar雄性大鼠随机分为正常组、模型组、氨溴索组、二陈汤组、蒌芩止嗽煎组各10只。用熏吸香烟加气管内注射内毒素及鼻腔滴入金黄色葡萄球菌,鼓风干燥箱中进行风热刺激的方法建立大鼠AECOPD痰热证模型,病理组织使用苏木精-伊红(HE)染色,应用图文分析软件(IPP6.0)测量肺平均内衬间隔(MLI)、平均肺泡数(MAN)、肺泡腔面积与总面积比(PAA);同时对Weigert染色标本测量弹力纤维的相对面积。[结果] 病理结果显示,AECOPD模型组与正常组比较肺组织结构严重受损,炎症细胞增多,说明模型复制成功,蒌芩止嗽煎组与氨溴索组受到破坏相对较轻,二陈汤组次之。MAN及PAA:蒌芩止嗽煎组、氨溴索组、二陈汤组与模型组相比较差异有统计学意义。Weigert染色显示,肺泡壁弹力纤维,其中以蒌芩止嗽煎组弹力纤维含量最高,以模型组受破坏最严重,其顺序从高到低依次为蒌芩止嗽煎组、氨溴索组、正常组、二陈汤组和模型组。[结论] 中西医化痰法对AECOPD痰热证大鼠模型受损肺组织结构均有较好的保护作用,以清热化痰法最为明显;清热化痰法提高抗生素的肺内转运作用的机制可能是通过不同程度改善肺组织受损的形态结构,降低受损肺泡结构对抗生素的渗透限制来协助抗生素在肺组织中的正相扩散,协同抗生素发挥作用。

Abstract:

[Objective] Based on the previous research foundation that the way of clearing heat and dispersing phlegm can increase the concentration of antibiotic in AECOPD lung tissue, this study will explore the mechanism of action that Louqinzhisoujian improves transport of antibiotics in the lung tissue by the means of intervening AECOPD damaged lung tissue morphology. [Methods] Fivty Wistar male rats were randomly divided into normal group, model control group, ambroxol group, Erchentang group, Louqinzhishoujian group,10 in each group . To establish rat model of AECOPD(syndrome of phlegm and heat) by passive cigarette smoking,intratracheal instillation of lipopolysacchricle(LPY), intranose instillation of staphylococcus aureus, plus put in the Wind-heat oven. Pathological tissue using HE staining, apply image analysis software(IPP6.0) measuring the average lung lining interval(MLI), mean alveolar number(MAN), alveolar area and the total area ratio(PAA); Weigert stained specimen while measuring relative area of elastic fibers. [Results] Pathology results showed : lung tissues structure of model control group were more severe damage than those of normal group, including increase of inflammatory cells. In this way the model successfully reproduced. The lung tissue destructions of Louqinzhishoujian group and ambrohexel group were relatively light, the second group followed Erchentang group. MAN and PAA: Compared with model control group, Louqinzhishoujian group, Erchentang group, and ambroxol group,they were statistically significant(P<0.05). Weigert staining showed the alveolar wall elastic fiber, Louqinzhisou group was the highest of elastic fiber content and model control group was the most serious damage among them, the orders from high to low were Louqinzhisou group, ambroxol group, normal group, Erchentang group and model control group.[Conclusion] The dispersing phlegm ways of TCM and WM also have better protection for damaged lung tissue of AECOPD rat model, the way of clearing heat and dispersing phlegm way is special evident. The mechanism of action that Louqinzhisoujian improves transport of antibiotics in the lung tissue may to some extent improve the damaged structure of pulmonary tissue to reduce the penetration of the antibiotic which helps it to play a role.