| 摘要: |
| [目的] 建立从龙胆碱性乙醇提取物中分离龙胆碱的高速逆流色谱(HSCCC)方法。[方法] 采用高速逆流色谱仪分离纯化龙胆碱性乙醇提取物中的龙胆碱,通过优化溶剂系统,以正己烷:乙酸乙酯:甲醇:水(5:5:4:6)为溶剂体系,上相为固定相,下相为流动相,仪器转速为850 r/min,流速为1.5 mL/min,温度为25 ℃,检测波长为280 nm.[结果] 龙胆碱性乙醇提取物经过D151弱酸性阳离子交换树脂富集(20%乙醇洗脱部分),再采用HSCCC技术进行分离纯化,制备得到的98.8%纯度的龙胆碱。[结论] 该方法稳定、回收率高,可高效地从龙胆碱性乙醇提取物中分离龙胆碱。 |
| 关键词: 龙胆 龙胆碱 高速逆流色谱 |
| DOI：10.11656/j.issn.1673-9043.2016.01.12 |
| 分类号: |
| 基金项目:天津市科技计划资助项目(12TXGCCX03800). |
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| HSCCC separation and purification gentian alkaline ethanol extracts of gentian |
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SHI Yao, WANG Lin-lin, YU He-shui, LAI Qing-hai, DING Hui, ZHANG Li-juan, SONG Xin-bo
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Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To establish the HSCCC method for separating the gentianine from the alkaline ethanol extracts of gentiana. [Methods] Separation and purification of gentianine by TBE300B HSCCC technology, through the optimization of the solvent system, with n-hexane : ethyl acetate : methanol : water (5:5:4:6) as the solvent system, the upper as stationary phase and the next phase as mobile phase, the rotational speed of the instrument was 850 r/min, the flow rate was 1.5 mL/min, the temperature was 25 ℃, the detection wavelength was 280 nm. [Results] The gentianine was obtained from the gentian alkaline ethanol extracts by D151 cation exchange resin combining with HSCCC, the content was above 98.8%. [Conclusion] The method is stable and has high results, it could be used to separate gentianine efficiently from alkaline ethanol extract of gentiana. |
| Key words: Gentiana scabra Bunge gentianine HSCCC |
