葛根素对大鼠酒精性肝纤维化的干预作用以及对肝星状细胞增殖活化的影响

Effects of puerarin on proliferation activation of hepatic stellate cell in rats with alcoholic liver fibrosis

关键词:  葛根素  酒精性肝纤维化  肝星状细胞  增殖细胞核抗原  α-平滑肌肌动蛋白

Key words:  puerarin  alcoholic liver fibrosis  hepatic stellate cell(HSC)  proliferating cell nuclear antigen(PCNA)  Alpha-smooth muscle actin(α-SMA)

摘要:

[目的] 研究葛根素对大鼠酒精性肝纤维化的干预作用以及对肝星状细胞增殖活化的影响。[方法] Wistar雄性大鼠85只被随机分为正常对照、模型、葛根素低、中、高剂量和阳性药(易善复)对照6组。除正常对照组之外,其余给予大鼠灌胃白酒辅以玉米油、吡唑的混合食料制备酒精性肝纤维化模型,12周后采用苏木精-伊红(HE)、Masson染色观察大鼠肝脏组织纤维化状况。采用免疫组化法检测大鼠肝组织内增殖细胞核抗原(PCNA)、α-平滑肌肌动蛋白(α-SMA)的表达,根据PCNA的表达,计算肝细胞的增殖指数。[结果] 模型组和各给药组肝纤维化程度显著高于正常对照组,各给药组肝纤维化程度要轻于模型组,但低剂量组肝纤维化程度重于其他各给药组。模型组肝细胞增殖指数明显高于其他各组,各给药组肝细胞增殖指数高于正常组。模型组α-SMA的表达高于其他各组,葛根素中、高剂量组和阳性药组α-SMA的表达低于模型组,葛根素低剂量组α-SMA的表达与模型组没有显著性差异,但从数据趋势上看低于模型组。[结论] 葛根素能通过抑制肝星状细胞的增殖活化从而达到防治酒精性肝纤维化的功效,以中、高剂量效果更明显。

Abstract:

[Objective] To research the intervention effect of puerarin on alcoholic liver fibrosis in rats and the effects on the proliferation activation of hepatic stellate cell. [Methods] Eight-five male wistar rats were randomly divided into normal group, model, low, medium and high puerarin-dose and effective medicine group, a total of 6 groups. Except the normal group, alcoholic hepatic fibrosis model was produced in rats by giving gavage with alcohol supplemented by corn oil, pyrazol. After 12 weeks, the status of hepatic fibrosis was observed by HE and Masson stain, The expression of PCNA, α-SMA in liver tissue was detected by immunehistochemical method. The proliferation index of liver cell was calculated by the expression of PCNA. [Results] The degree of liver fibrosis of the model group and each medication administration group were significantly higher than the normal group, and the degree of liver fibrosis of each medication administration group was lighter than the model group. But the degree of liver fibrosis of the low-dose group was more serious than any other medication administration group .The proliferation index in the model group was higher than any other groups, and the proliferation index in normal group was lower than the other groups. The expression of α-SMA in the model group was higher than any other. The expression of α-SMA in the medium puerarin-dose group, the high puerarin-dose group and the effective medicine group were lower than the model group. There was no significant difference between the expression of α-SMA in the low puerarin-dose group and the model group. [Conclusion] The efficacy of peurarin in the prevention and treatment of alcoholic hepatic fibrosis are implemented by inhibiting the proliferation and activation of hepatic stellate cell in rats, and the effects of the medium and the high dose group are more significant.