| 摘要: |
| [目的] 获取药用动物乌梢蛇线粒体基因组全序列。[方法] 采用Ion Torrent PGM技术，对乌梢蛇全基因组DNA进行提取测序，采用MIRA方法从头组装方法和近缘种mapping方法进行组装拼接，应用MITOS等方法进行注释。[结果] 获得17 162 bp乌梢蛇线粒体基因组全序列。其共编码22个tRNA，13个蛋白编码基因，2个rRNA（16S rRNA、12S rRNA）并有1个轻链（L链）复制起始区。[结论] 获取乌梢蛇线粒体基因组全序列，并可用于该药用动物的资源调查与保护研究。 |
| 关键词: 乌梢蛇 线粒体基因组 线粒体DNA 序列分析 |
| DOI：10.11656/j.issn.1673-9043.2016.03.10 |
| 分类号: |
| 基金项目:国家自然科学基金项目（81202874）；高等教育博士学科点专项科研基金（20121210120006）；国家科技支撑计划项目（2012BAI29B01）。 |
|
| Analysis of mitochondrial genome of Ptyas dhumnades |
|
LIU Jie, TIAN Xiao-xuan, CUI Ying, ZHU Yan
|
|
Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China
|
| Abstract: |
| [Objective] To investigate the complete mitochondrial genome of medical animal Ptyas dhumnades (Cantor, 1842). [Methods] The Ion Torrent PGM technique was chosed to sequence genomic DNA of Ptyas dhumnades, and then the strategy of de novo assembly and mapping versu mtgenome of closely related species by MIRA 4.0 was adopted to get the complete mitochondrial sequence. This genome was annotated by Online server MITOS etc. [Results] The 17 162 bp complete mitochondrial genome of Ptyas dhumnade with high coverage contains 22 transfer RNA, 13 protein coding genes, 2 ribosomal RNA genes and 1 putative L-strand replication origin. [Conclusion] The mtgenome of Ptyas dhumnades was obtained, and could be furher applied in the resources survey and protection of this medical animal. |
| Key words: Ptyas dhumnades mitochondrial genome mitochondrial DNA sequence analysis |
