| 摘要: |
| [目的] 建立同时测定羌活酚酸（新绿原酸、绿原酸和阿魏酸）和香豆素（羌活醇和异欧前胡素）两类成分的高效液相色谱-紫外分析法（HPLC-UV），为羌活药材的质量评价提供技术支撑。[方法] Agilent Zorbax SB-C18反相色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.2%磷酸水，检测波长330 nm，进样量5 μL，流速1 mL/min。[结果] 两类成分具有良好的线性关系，方法学验证均符合要求。[结论] 该方法简单易行，重复性好，专属性强，可用于羌活药材的质量控制。 |
| 关键词: 羌活 高效液相色谱-紫外分析法 含量测定 |
| DOI：10.11656/j.issn.1673-9043.2016.03.11 |
| 分类号: |
| 基金项目:国家自然基金青年项目（81503213）；天津市高等学校创新团队培养计划资助（TD12-5033）。 |
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| Simultaneous determination of phenolic acids and coumarins for quality control of Rhizoma et Radix Notopterygii.by HPLC-UV |
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YANG Xi, GONG Mei-ling, LI Jin, JIN Hua, MA Lin, CHANG Yan-xu
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Tianjin State Kay Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] An HPLC-UV method was established to simultaneously determine the content of phenolic acids (neochlorogenic acid, Chlorogenic acid, Ferulic acid) and coumarins (Notopterol, Isoimperatorin) for the quality control of Rhizoma et Radix Notopterygii. [Methods] An Agilent Zorbax SB-C18 column (4.6 mm×250 mm, 5 μm) with a guard column was used as the analytical column to separate the multi-ingredients. The mobile phase was acetonitrile and 0.2% (V/V) phosphoric acid solution. The flow rate was set at 1 mL/min. The injection volume was 5 μL and the eluent was measured at 330 nm. [Results] This method exhibited a good linear relationship, and the results of the method validation up to the requirements. [Conclusion] The established HPLC-UV assay could be used to determinate the content of Rhizoma et Radix Notopterygii for the quality control. |
| Key words: Rhizoma et Radix Notopterygii. HPLC-UV content determination |
